TOTAL BILE ACIDS FIFTH GENERATION AN ENZYMATIC COLORIMETRIC METHOD FOR BILE ACID TESTING

TOTAL BILE ACIDS THE FIFTH GENERATION TEST The fifth generation bile acids test offered by Randox is a 2 shot liquid, enzymatic colorimetric assay for the quantitative in vitro determination of bile acids in serum or EDTA Heparinized plasma. The fifth generation assay incorporates a more complex enzyme cycling method compared to previous generations to amplify the signal: ultimately improving sensitivity and precision while also reducing interference from haemolytic and lipaemic samples. Haemolysis and lipaemia is common in neonate and pregnant patients, making the fifth generation test ideal...

TOTAL BILE ACIDS ASSAY PRINCIPLE Two reactions are combined in this kinetic enzyme cycling method. In the first reaction, bile acids are oxidised by 3-α hydroxysteroid dehydrogenase with the subsequent reduction of Thio-NAD to Thio-NADH. In the second reaction, the oxidised bile acids are reduced by the same enzyme with the subsequent oxidation of NADH to NAD. The rate of formation of Thio-NADH is determined by measuring the specific absorbance change at 405nm. Enzyme cycling means multiple Thio-NAD molecules are generated from each bile acid molecule giving rise to a much larger absorbance change,...

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