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Total Bile Acids
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Total Bile Acids - 1

TOTAL BILE ACIDS FIFTH GENERATION AN ENZYMATIC COLORIMETRIC METHOD FOR BILE ACID TESTING

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TOTAL BILE ACIDS THE FIFTH GENERATION TEST The fifth generation bile acids test offered by Randox is a 2 shot liquid, enzymatic colorimetric assay for the quantitative in vitro determination of bile acids in serum or EDTA Heparinized plasma. The fifth generation assay incorporates a more complex enzyme cycling method compared to previous generations to amplify the signal: ultimately improving sensitivity and precision while also reducing interference from haemolytic and lipaemic samples. Haemolysis and lipaemia is common in neonate and pregnant patients, making the fifth generation test...

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TOTAL BILE ACIDS ASSAY PRINCIPLE Two reactions are combined in this kinetic enzyme cycling method. In the first reaction, bile acids are oxidised by 3-α hydroxysteroid dehydrogenase with the subsequent reduction of Thio-NAD to Thio-NADH. In the second reaction, the oxidised bile acids are reduced by the same enzyme with the subsequent oxidation of NADH to NAD. The rate of formation of Thio-NADH is determined by measuring the specific absorbance change at 405nm. Enzyme cycling means multiple Thio-NAD molecules are generated from each bile acid molecule giving rise to a much larger absorbance...

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Copyright © 2021 Randox Laboratories Ltd. All rights Reserved. VAT number: GB 151682708. Product availability may vary from country to country. Some products may be for Research Use Only. For more information on product application and availability, please contact your local Randox Representative

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