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Randox Laboratories
Total Bile Acids

Total Bile Acids

Product catalog summary

Clinical Significance of Bile Acids
Testing serum bile acids is crucial for diagnosing and prognosing liver diseases, as it reflects liver function rather than damage. Abnormal bile acid levels can indicate liver disease, impaired liver function, intestinal dysfunction, gall bladder blockages, or obstetric cholestasis during pregnancy. Intrahepatic cholestasis of pregnancy (ICP) is a specific liver disorder during pregnancy, marked by elevated bile acids, which can significantly increase the risk of stillbirth and preterm labor.

Biological Significance of Bile Acids
Bile acids, derived from cholesterol metabolism in the liver, are essential for digesting and absorbing fats and fat-soluble vitamins. They perform five key physiological functions: cholesterol elimination, lipid transportation, bile flow stimulation, biliary phospholipid secretion stimulation, and negative feedback regulation of bile acid and cholesterol biosynthesis.

The Fifth Generation Test
The Randox fifth generation bile acids test is a 2-shot liquid, enzymatic colorimetric assay for measuring bile acids in serum or plasma. It uses an advanced enzyme cycling method to enhance sensitivity and precision, reducing interference from haemolytic and lipaemic samples. This test is suitable for neonate and pregnant patients due to its smaller sample volume requirement.

Ordering Details
The test kits are available in different sizes, with specific catalog numbers for easy ordering. Controls and calibrators are also available to ensure comprehensive testing.

Key Features
The test offers superior sensitivity and precision, a wide measuring range, excellent correlation with other methods, and is stable when stored properly. It is convenient and easy to use, with applications available for various clinical chemistry analyzers.

Assay Principle
The assay uses a kinetic enzyme cycling method involving two reactions. Bile acids are oxidized and reduced, with the formation of Thio-NADH measured by absorbance change at 405nm, enhancing assay sensitivity.

Catalog excerpts

TOTAL BILE ACIDS FIFTH GENERATION AN ENZYMATIC COLORIMETRIC METHOD FOR BILE ACID TESTING

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TOTAL BILE ACIDS THE FIFTH GENERATION TEST The fifth generation bile acids test offered by Randox is a 2 shot liquid, enzymatic colorimetric assay for the quantitative in vitro determination of bile acids in serum or EDTA Heparinized plasma. The fifth generation assay incorporates a more complex enzyme cycling method compared to previous generations to amplify the signal: ultimately improving sensitivity and precision while also reducing interference from haemolytic and lipaemic samples. Haemolysis and lipaemia is common in neonate and pregnant patients, making the fifth generation test ideal...

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TOTAL BILE ACIDS ASSAY PRINCIPLE Two reactions are combined in this kinetic enzyme cycling method. In the first reaction, bile acids are oxidised by 3-α hydroxysteroid dehydrogenase with the subsequent reduction of Thio-NAD to Thio-NADH. In the second reaction, the oxidised bile acids are reduced by the same enzyme with the subsequent oxidation of NADH to NAD. The rate of formation of Thio-NADH is determined by measuring the specific absorbance change at 405nm. Enzyme cycling means multiple Thio-NAD molecules are generated from each bile acid molecule giving rise to a much larger absorbance change,...

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Copyright © 2021 Randox Laboratories Ltd. All rights Reserved. VAT number: GB 151682708. Product availability may vary from country to country. Some products may be for Research Use Only. For more information on product application and availability, please contact your local Randox Representative

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Total Bile Acids

Total Bile Acids

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