ECM-BioInk Datasheet
3Pages

PeptiGel Design '% regenHU BIOSYSTEM ARCHITECTS Type ECM-a BioInk™ ECM-BiolnkTM Datasheet1. PRODUCT DESCRIPTION ECM-BioInk™ is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from ECM-BioInk™ fluid precursors into highly intertwined hydrated peptide hydrogel fibres when presented with ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the ECM-BioInk™ solution, researchers can control the flexibility of the 3-D architecture to meet their applications specific needs. ECM-BioInk™ is the unique human relevant synthetic extracellular matrix where scaffold interaction with cells and small molecules is driven by hydrogen and hydrophobic interactions. We offer an ECM-BioInk™ portfolio with 5 formulations that differs from the cell-scaffold interactions (scaffold positive electrical net charge ranging from 0 to +2) as well than the simple tuning of the synthetic extracellular-matrix mechanical strength. Cell response to material depends on a number of variables which include: Cell type (line of stem), nature of material (functional or non-functional) as well as cell response targeted (spheroids vs. sheets). As a result the matrix requirement will vary. ECM-BioInkTM technology platform can be further functionalized with further biological and chemical cues to develop the perfect printable matrix for your application. Electrical charge: medium (M) v/W ratio: pH: G' (24h in PBS): Electrical charge: v/W ratio: pH: G' (24h in PBS): Electrical charge: v/W ratio: pH: G' (24h in PBS): Electrical charge: v/W ratio: pH: G' (24h in PBS): Electrical charge: regenHU Ltd. | Z.I. du Vivier 22 | 1690 Villaz-St-Pierre | Switzerland | P +41 26 653 72 20 | F +41 26 653 72 21 | biomaterials@regenhu.com | www.regenhu.com

PeptiGelDes/gn % regenHU ^ BIOSYSTEM RRCHITECTS 2. INTENDED USE ECM-BioInk™ has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, cardiomyocytes, osteoblasts and chondrocytes. A selection table can be found below. Fibroblasts Human Embryonic Stem Cells Human Induced Pluripotent Stem Cells Human Mesenchemical Stem Cells Bone Marrow Derived Mesenchemical Stem Cells Adipose Tissue Derived Mesenchemical Stem Cells Human Haemopoetic Stem Cells _c o in i o LU x x x x x x x Human...

Manual Mixing with Laboratory Tools - Tools needed (all should be sterile): - Micro-spatula - Cells suspension - Small container fox mixing (e.g. petri-dish) - A cartridge and a piston Harvest the cells and count them. We advise a cell concentration of approximately 20-1 06cells/ml - The mixing ratio is 1:1 0. For 1 ml of ECM BioInk™ mix 100 gl of cell suspension in media - Dispense the two amounts together and gently mix them with the micro spatula until you obtain a homogenous suspension. - Fill the cartridge with the mixed suspension. Push to the bottom of the cartridge with the...