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Renishaw’s RA816 biological analyser is a compact benchtop Raman imaging system that redefines tissue and biofluid analysis. It is easy to use, yet gives rich biochemical information about the biological samples being analysed. Designed exclusively for the biological sciences community, the system rapidly obtains detailed information on the distribution and amount of biochemical species within biological samples, including tissue biopsies, tissue sections and biofluids. The RA186 biological analyser brings together the chemical analysis power of Raman spectroscopy (a light scattering technique)...

Detailed biochemical information is revealed from biological samples. From the distribution of exogenous and endogenous compounds within tissue, to the detection of protein secondary structure changes due to drug interaction and tissue injury.

• Easy to use software with simple concept and workflow: making Raman spectroscopy accessible to all users, without compromising performance. • Macro and high magnification imaging modes, with digital zooming for a range of sample sizes. • Reflection or transmission illumination for best image contrast. • Image tiling for large area coverage. • Small footprint - ideal for a space-conscious laboratory environment. • System can be easily moved between lab and clinic with inbuilt performance checking to ensure accuracy. • Robust system that does not require user alignment. • Stable and repeatable,...

Proteins Lipids Nucleic Acids Proteins Carbohydrates Nucleic acids Tyrosine Phenylalanine A typical biological tissue Raman spectrum. It demonstrates the wealth of information obtained from a single measurement.

Powerful software Data acquisition Building and validating a classification model The RA816 biological analyser is entirely computer-controlled. Its software provides a structure for managing every step of the measurement process using pre-defined experiment setups and protocols. The unique macro image provides a comprehensive overview of all subsequent work and enables easy sample navigation and visualisation. The system includes a software package that enables the user to: • Process spectral datasets. • Build, test and validate pathology and disease classification models (using PCA-LDA). •...

Imaging human brain tissue. Comparison of (a) white light and (b) Raman-false-coloured composite image of a cerebellum whole follicle, showing arbor vitae / white matter (red), granule cell layer (green), molecular layer (dark blue) and meninges (pia, arachnoid and dura mater) (cyan).

Example workflows for routine operation Raman tissue imaging with the RA816 biological analyser and correlating results with stained or labelled slides Step one Place your stained or labelled slide in the dedicated holder and select from a list of available bio-sample types. Step two The system automatically digitises the slide using reflection or transmission white light imaging (macro view or high-resolution tile). Unstained white light image. Step three Insert and digitise an adjacent unstained tissue section. Step four Select the whole of the unstained sample for Raman analysis. Or select...

Mean of example spectral dataset. Example: Multivariate analysis (PCA-LDA) of the IDH genomic mutation status of brain glioma. Example: Sensitivity and specificity for predicting genetic subtypes using pc-linear discriminant analysis (LDA) 3-group and 2-group models for fresh tissue samples, cryosections, and formalin-fixed paraffin-embedded (FFPE) sections*1 *' Laurent James Livermore et al, Rapid intraoperative molecular genetic classification of gliomas using Raman spectroscopy, Neuro-Oncology Advances, Volume 1, Issue 1, May-December 2019, vdz008, https://doi.org/10.1093/noajnl/vdz008

apply innovation™ The RA816 biological analyser is designed for research use only (RUO) and is not for use in diagnostic procedures. © 2021 Renishaw plc. All rights reserved. RENISHAW® and the probe symbol are registered trade marks of Renishaw plc. Renishaw product names, designations and the mark ‘apply innovation’ are trade marks of Renishaw plc or its subsidiaries. Other brand, product or company names are trade marks of their respective owners. Renishaw plc. Registered in England and Wales. Company no: 1106260. Registered office: New Mills, Wotton-under-Edge, Glos, GL12 8JR, UK. WHILE CONSIDERABLE...