KrosFlo® KR2i RPM™ System Product Specifications Specification Sheet Overview The KrosFlo® KR2i RPM™ System integrates Real-time Process Management with lab-scale Tangential Flow Filtration (TFF). The system combines the KrosFlo® KR2i System and the CTech™ FlowVPX® Variable Pathlength UV-Visible spectrophotometer to provide automated TFF with in-line concentration monitoring and end point control. By coupling the KR2i and FlowVPX functionality, the system delivers improved process control and efficiency with reduced process risk. • Strengthen process control with high quality and highly reproducible concentration results. Increase process efficiency by reducing cycling time, increasing yield, throughput, and eliminating the need for time consuming off-line pre and post run concentration measurements and dilution. Reduce process risk by ensuring accurate concentration throughout the TFF process. Automation and Modernization The KrosFlo KR2i System provides automated, modern, flat-sheet TFF capabilities from 140 ml to 15 L. Modular plug-and-play hardware components, configurable ProConnex® Flow Paths, and graphically driven software ensure optimal performance and ease of use. The system is driven by a Quattroflow® diaphragm pump, which supports high-pressure processes up to 4 bar. Real-Time Concentration Insights The FlowVPX in-line spectrophotometer uses variable pathlength technology to monitor and optimize critical process parameters in real-time for improved quality and consistency. Slope-based concentration measurements increase the efficiency of the process and avoid costly dilution and background correction steps, revealing process characteristics previously hidden from traditional, fixedpathlength sensors. In-line measurements enable real-time, automated decisions, potentially eliminating out-of-spec results and costly, time-consuming deviation reports. Robust Control Software The proprietary KrosFlo® RPM™ Software enables the combined functionality of the KR2i and FlowVPX systems. The software can execute complicated TFF processes using real-time concentration data through user-specified set points for the system auxiliary pumps, scales, backpressure valve, and FlowVPX system. This level of system programmability results in improved accuracy, method reproducibility, and process efficiency. Quattroflow® is a registered trademark of PSG. repligen.com © 2023 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property of Repligen Corporation and/or its affiliate(s) or their respective owners. | DOC0302

Specification Sheet KrosFlo® KR2i RPM™ System Filter Mode Hollow Fiber Flat Sheet Cassettes Recommended Process Volume Filter Surface Area 13 cm2-3700 cm2 Main Drive / Feed Pump and Stand Easy-Load Pump Head - up to 2.3 LPM (600 RPM) Use with tubing sizes #13, #14, #16, #17, #18 and #25. Integrated stand for hollow fibers Automatic Backpressure Control Valve (ABV) Controls user-set process TMP or permeate pressure Tablet as HMI Microsoft® Surface Pro® with Krosflo® RPM™ control and data acquisition software Auxiliary Pump KrosFlo® Junior, flowrates up to 0.3 LPM System Scales Readability: 0.1...

KrosFlo® KR2i RPM™ System Specification Sheet KONDUiT Add-On Monitor 2 Temperature Inputs Sensor Size 1/4" HB and 1/2" HB Conductivity Range 0.1 to 100 mS/cm Conductivity Accuracy 2 mS/cm-50 mS/cm: ±5% Temperature Accuracy ±0.2°C or better Auxiliary Components Up to 3 single-use sensors Pressure transducer Pressure Range: -7 to 75 psi (-0.48 to 5.2 bar) Polysulfone, luer-style ports Tubing PharmaPure, TPE, size #14 and #16 Stand Integrated stand with hollow fiber holder Auxiliary Component Octopus cable Allows for connectivity of scales, auxiliary pumps, and KONDUiT System 15 mL, 50 mL, 250 mL,...

Specification Sheet KrosFlo® KR2i RPM™ System Auxiliary Pumps (2): KONDUiT: KR2i Main pump Auxiliary Pumps (ea) Konduit FlowVPX (3 mm flow cell): Low Voltage Directive 2014/35/EU CE Mark Electromagnetic Compatibility Directive 2014/30/EU RoHS Directive 2011/65/EU Environmental Compliance REACH Regulation (EC) No. 1907/2006) Support and Training: Repligen is committed to customer success from predelivery through installation and training. Included with purchase: • Full 12-month warranty support • Post-obsolescence seven-year hardware support • Single- and multi-year service contract options, which...

KrosFlo® KR2i RPM™ System Specification Sheet Customer Support (cont.) More information: Final application suitability of all materials and ratings are the sole responsibility of the user. Specified pressure and temperature ratings may be subject to limitations. Contact a Repligen analytical sales representative for more information. C Technologies, Inc. and/or its affiliates, to the extent allowed by law, disclaims, and in no event shall be liable for, any incidental or consequential damages in connection with user, instrument, or system performance in relation to all content contained in this...