PRRS antibody ELISA Kit
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PRRS antibody ELISA Kit PRRS Ab Test Product Number: E30021 Product Unit: 1 plate, 96T / 2 plates, 192T / 5plates, 480T 1. Introduction 2. Description of Test 3. Precautions 4. Limitations of Test 5. Reagent Provided 6. Instrument Required 7. Reagent Preparation 8. Sample Preparation 9. Assay Procedure 10. Result Determination 11. Storage and expiration 12. References Manufacturer Information Ring Biotechnology Co., Ltd E-mail: export@ringbio.com Add: Building 3, Zhongtongtai TechnoPark, No. 11, Kechuang 14th St, Beijing 100176, CHINA Tel: +86-10-56267496 Technical Support & Service: +86-13811393460 1/4 For research use only. Not for use in diagnostic or therapeutic procedures.

1. Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a virus that causes a disease of pigs, called porcine reproductive and respiratory syndrome (PRRS), also known as blue-ear pig disease. This economically important, panzootic disease causes reproductive failure in breeding stock and respiratory tract illness in young pigs. The disease costs the United States swine industry around $644 million annually, and recent estimates in Europe found that it costs almost 1.5 billion euro every year. This kit utilizes recombinant nucleocapsid protein of PRRSV(protein N),...

The kit contains the following items. Item No. Microplate pre-coated with PRRS antigen Positive Control Negative Control Sample buffer Stop solution Note: Stop solution is 2M sulfuric acid, which is not provided due to air cargo shipment, which shall be prepared by client. 6. Instrument Required  ELISA reader Wash buffer: dilute the 25xWash buffer provided in the kit with deionized water in the volume ratio of 1:24. For example, 1ml 25xWash buffer + 24ml deionized water The diluted wash buffer can be stored at room temperature for 2 weeks. No other reagent is required. Please remember to...

7) Incubation: cover the plate with plate cover and incubate at room temperature (22-27 oC) for 30min. 8) Washing: pour the liquid out from the wells and wash with wash buffer (300ul per well) for 3 times. Tap the residue liquid against absorbent paper to make sure the plate is dry after washing. 9) Add enzyme conjugate: add 100ul of HRP conjugate into each well. Cover the plate again and then incubate at room temperature again for 30min. 10) Washing: repeat the washing step again. 11) Add substrate: add TMB substrate into each well, 100ul per well. Cover the plate again and then incubate...