AVENIO Edge System Elevating NGS sample prep automation

Transform your laboratory with true walk away automation The AVENIO Edge System is a fully automated system for library preparation, target enrichment and quantification. Experience high quality results from absolute automation, thanks to validated protocols, integrated components and end-to-end control. Finally, laboratory personnel have the freedom to walk away and work away, with confidence and trust. Overcome the barriers of manual sample preparation Even the most advanced laboratories are constrained by the number of steps still being performed manually, making work labor-intensive, time-consuming,...

Redefining Simplicity Maximizing Productivity Empowering Trust

Redefining simplicity Streamline daily routines with virtually touchless sample preparation The AVENIO Edge System minimizes manual touchpoints with all-in-one hardware, ready-to-run reagents and simple workflow management software. This powerful system is designed for effortless operation that anyone in the laboratory can use. Instrument touchscreen shows visual progress of your workflow with estimated end-run times Automatic load-check of consumables One-box automation with quantification, thermal cycling, and pooling on-board *Pre-capture workflow comparison with manual KAPA Hypercap v3 data...

Maximizing productivity Optimize laboratory resources by alleviating demands on staff The AVENIO Edge System increases laboratory efficiency by automating tedious, time-intensive processes that are usually performed manually. Highly qualified laboratory staff can now be freed of constant instrument surveillance to focus on other important tasks. • Zero manual pipetting for routine use • Set up to walk away in less than 20 minutes • Reliable walk away automation for up to two days samples end-to-end in ~36 hours* *For library preparation, pooling and target enrichment.

Empowering trust Produce accurate, reliable and reproducible results The AVENIO Edge System provides unmatched sensitivity, ensures significantly less risk of cross-contamination and carryover and reduces errors. Run any sample type from a wide range of sample inputs including plasma, cell lines and whole blood, with quality results every time. • High sensitivity due to proprietary advanced bead-processing technology • Powered by proven and trusted KAPA reagents* • Excellent on-board reagent stability for ≥ seven days • Integrated pre-analytical system checks to help prevent costly errors *For...

Hybridization-based capture workflow performance was comparable between automated and manual workflows Optimal balance between uniformity and specificity (on-target) with acceptable results over 90% of the time across different runs. Automated and Manual Runs Comparison 100% AVENIO Edge System workflow study used baseline library preparation/target enrichment conditions and were sub-samples to 60M reads.* Average Median Target Coverage Automated HyperExome Comparable results across all samples between the gold standard used in manual workflows (Qubit) and the AVENIO Edge DNA Quantification kit....

Innovation and quality only from Roche Exclusive features that set the AVENIO Edge System apart Fully integrated on-deck thermal cycler streamlines sample amplification Proprietary advanced beadprocessing station provides unparalleled sensitivity and yield Tip park reduces reloading time, risk of contamination and need for disposables Powerful AVENIO Designer Software offers coding-free protocols and flexible configuration of user-defined workflows Fully barcoded reagents and consumables are automatically loaded and calculated, reducing waste and increasing accuracy Integrated, automated quantification...

Barcode reader Piercing tool Storage compartment Gripper finger exchange stations Waste management system for solids and liquids Status light indicator Cooling station Temperature-controlled heater/shaker Hand-held barcode scanner 13 Quantification module 14 Thermal cycler 15 Gripper arm Efficient footprint maximizes your laboratory space Beckman Biomek i7 1700mm x 810mm (66.9 in x 31.9 in) Perkin Elmer Sciclone NGS Workstation 1219mm x 711mm (48 in x 28 in) AVENIO Edge

Software that seamlessly integrates into your laboratory for easy workflow management at every step AVENIO Edge Software Powerful software comes standard with the AVENIO Edge System for smooth, reliable operation and workflow guidance. • No special training required for staff—easy step-by-step instructions are provided • Consumables list is displayed prior to every run for efficient resource management • Touchscreen monitor works even if operator is wearing gloves • Visual progress of the workflow and samples is available at a

AVENIO Designer Software* Intuitive, templated design software for rapid and flexible user-defined workflow deployment. Easy-to-use interface with tunable parameters Unparalleled flexibility allows changing workflows at any time Workflows can be configured to stop or start at any step Validated workflows are pre-populated with recommended parameters and the ability to adjust if needed * User-defined protocols created using AVENIO Designer Software are for research use only workflows, not for use in diagnostic procedures.

The foundation for an end-to-end future As demands change, adaptability is a key attribute for any laboratory. The AVENIO Edge System can function as a powerful component in a single- or multi-room configuration—yet it is also the foundation for an all-inclusive standalone system. Install it today for day-one productivity, and build on it as the future of next-generation sequencing develops. Nucleic acid extraction Library preparation Target enrichment Library quantification Analysis & reporting With Roche legacy, service and support, we will be with you to keep your laboratory moving forward....