KAPA EvoPrep Kits KAPA EvoPrep Kits: every breakthrough begins with brilliant sample prep. The KAPA EvoPrep Kits are the latest high-performance, streamlined, and automation-friendly library preparation solutions from Roche. The kits are validated with challenging sample inputs such as cell-free DNA, FFPET DNA, or mechanically sheared DNA, and developed to help reduce workflow steps. Their ReadyMix reagents are available in tube and plated formats for enhanced automatability and include the KAPA EvoT4 DNA Ligase (in the ligation ReadyMix). This new evolved ligase is specifically engineered to increase library conversion rates. These and the many other advanced features of the KAPA EvoPrep Kits were thoughtfully designed to set new standards in NGS sample prep workflows and jumpstart the journey to your next big research breakthrough. KAPA EvoPrep Kits offer a complete library preparation solution when combined with KAPA Adapters and KAPA HyperPure Beads (sold separately) and a complete target enrichment solution when combined with KAPA HyperCap or KAPA HyperPETE workflows. The kits are compatible with the Illumina sequencing platform and have been qualified with automation methods. Benefits of the KAPA EvoPrep Workflow* Simplified and streamlined workflow Simplified, streamlined and automatable workflow with ReadyMix reagents, available in tube and plated format to increase efficiency and convenience Exceptional library yields and sequencing quality Designed to provide increased library conversion efficiency with the KAPA EvoT4 DNA Ligase in the Ligation ReadyMix, enabling higher sensitivity and more confident variant detection Enable superior performance and sequencing efficiency Optimal utilization of sequencing throughput with minimal sequence coverage bias, minimization in the presence of sequencing artefacts with more unique library molecules recovered Constantly evolving, efficient, and complete solutions Nucleic Acid Extraction Library Preparation *Compared to earlier KAPA DNA Library Prep chemistries. Data on file. For Research Use Only. Not for use in diagnostic procedures. Target Enrichment Library Quantification Sequencing Library Sequencing Secondary Analysis

Simplified and streamlined workflow* Fragmented Input DNA The KAPA EvoPrep Kit provides a simplified and streamlined workflow to remove the complexities and risk for human error by providing a trusted library preparation solution. • Analyze more samples with a wide range of DNA inputs (0.1 ng-500 ng) • PCR-free workflows starting from 50 ng of DNA input Adapter Ligation* • ReadyMix formulations, therefore fewer reagents and hands-on-time • Tube and plated format increase efficiency and convenience Bead Cleanup* • Manual and automation friendly protocol • Ligation time of just 5 minutes, shortening...

• Improved sequencing metrics, allowing higher confidence in data due to reduction in sequencing artefacts KAPA EvoPrep Figure 4: Improved sequencing performance - uniform coverage with reduction in sequencing artefacts. PCR-free whole genome libraries were prepared using 100 ng of Covaris-sheared human genomic DNA (NA12878) with the KAPA EvoPrep Kit, Supplier I, Supplier Q and Supplier W, following each supplier's instructions for use. (A) The KAPA EvoPrep Kit had the lowest percentage of Chimeras present, resulting in higher data confidence2 compared to Supplier I, Supplier Q and Supplier W...

* KAPA Library Amplification Primer Mix (10X) not included. **KAPA HiFi HS RM and KAPA Library Amplification Primer Mix (10X) not included. *** Virtual kits. 1. McNulty, et al. (2020). Impact of reducing DNA input on next-generation sequencing library complexity and variant detection. The journal of Molecular Diagnostics, Volume 22, Issue 5, May 2020, Pages 720-727. 2. Chen, et al. (2024). Characterization and mitigation of artifacts derived from NGS library preparation due to structure-specific sequences in the human genome. BMC Genomics 25:227. I Learn more at sequencing.roche.com Roche Sequencing...