KAPA PROBE FORCE
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Product Summary KAPA PROBE FORCE Evolved to break through KAPA PROBE FORCE is our most inhibitor–resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Analyze crude samples that have comparable accuracy, reproducibility and sensitivity as samples derived of purified DNA using KAPA PROBE FORCE. • Direct qPCR from crude blood, tissue, and plant extracts • Sample-to-Cq workflows in <1 hour • High efficiency for accurate, reproducible, and sensitive results • Superior tolerance to carry-over inhibitors • Multiplex compatibility with crude extracts Data on file. For Research Use Only. Not for use in diagnostic pr

Streamline sample-to-Cq workflows KAPA PROBE FORCE enables the use of rapid crude DNA extraction methods and overcomes carry-over inhibitors. Competing master mixes used in traditional blood, tissue, and plant qPCR workflows require robust upstream sample processing (e.g., column purification or nuclease digestion). • Eliminate the time and cost of sample purification by amplifying directly from crude samples • Analyze a wide range of sample types including whole blood, cells, mouse tails, FFPE, leaf, stem, seed, and soil KAPA PROBE FORCE Traditional qPCR workflows QIAamp DNA Blood Mini (20...

KAPA PROBE FORCE exhibits consistent and robust amplification across all inhibitors tested, without observable Cq delays. • Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samples • Convert purified DNA assays to crude workflows without a loss in data quality <1 ACq ^ 1 - 2 ACq 2 - 3 ACq ^ >3 ACq • Detection failed. Lowest concentration at which Cq <45 cycles detected Figure 2. Broad range of high inhibitor resistance. Baseline performance of KAPA PROBE FORCE and competing master mixes was measured by creating standard curves with purified DNA according to each...

Multiplex crude Samples efficiently A: Cotton seed B: Grapevine discrimination of crude Mouse blood C: DNA extracts • Accelerate genotyping analysis with single reaction allelicleaf 4 4 • Maximize data collection from precious samples, increase throughput, and reduce costs Figure 3. Crude sample duplex SNP detection. KAPA PROBE FORCE provides accurate genotyping and tight clustering in the presence of crude extracts (A) cotton seed, 0.5M NaOH extraction; (B) grapevine leaf, 75 mM Tris-HCl and 5 mM TCEP extraction; (C) mouse blood, FTA 0.5 mm disc; and (D) mouse tail extracts, NaOH...