KAPA Taq PCR Kits
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Portfolio Overview High quality and performance for routine PCR KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart DNA Polymerase have 5'g3' polymerase and 5'g3' exonuclease activities, but no 3'g5' exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. Kits are ideally suited for: • standard PCR • DNA labelling • DNA sequencing • numerous applications for which a high-quality, thermostable DNA polymerase is required High performance • Improved sensitivity, specificity, and yields • Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product KAPA Taq HotStart Figure 1. Improved sensitivity, specificity, and yield. A 500 bp fragment of the CCR5 gene was amplified using 100 ng, 10 ng, 1 ng, or 100 pg of human genomic DNA as template. KAPA Taq HotStart exhibits improved sensitivity, specificity, and yield when compared with competitive HotStart products. All reactions were performed using the manufacturer recommended protocols. Data on file. For Research Use Only. Not for use in diagnostic procedures. Figure 2. Taq and KAPA Taq HotStart. A 270 bp amplicon was amplified from mycoplasma DNA with KAPA Taq or KAPA Taq HotStart. Sensitivity was tested using a 10X template dilution ser

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