Description: AccuSignal™ E. coli HCP ELISA Kit - KJB-4003 Applications: ELISA Host Species: Rabbit Background: The antibodies in the kit have been specifically designed to detect HCP contaminants and to measure analytes from crude cellular harvests to process intermediates. The E. coli ELISA Kit uses purified antibodies generated against a mixture of proteins of mock induced E. coli cell lysate. These antibodies react strongly with E. coli cell lysates from a commonly used E.coli strain, BL21. The key component of this kit is a broad coverage antibody produced against a spectrum of host cell proteins including both low abundancy and high abundancy proteins. Synonyms: host cell proteins, ELISA, process development Host Species: Rabbit Clonality: Polyclonal Detection Kit Type: ELISA Kit Detection Range: 2.6 - 250.0 ng/mL Purity/Specificity: This kit contains: E. coli-HCP antibody coated 96-well strip plate, Plate Sealer, E. coli-HCP standard, E. coli-HCP detection antibody, sample buffer, Wash Buffer, TMB Buffer, Stop Buffer, and protocol. Relevant Links: • KJB-4003 Protocol

Tested Applications: ELISA Application Note: The AccuSignal™ E. coli HCP ELISA Kit is a sandwich, enzyme-linked immunosorbent assay. The impurities in process-derived samples containing E. coli proteins are captured and detected in a microtiter-type plate by using pre-coated, purified, rabbit polyclonal, E. coli-HCP-specific antibodies. The immunological reaction after the addition of TMB results in a chromogenic change that can be measured photometrically at 450 nm with reference wavelength at 630-650 nm. The O.D. in the wells is directly proportional to the amount of HCP concentration and correlates...

Order online now! www.rockland.com [email protected] +1 484.791.3823 ELISA Accusignal™ E. coli HCP ELISA Kit and Components for ELISA ELISA The AccuSignal™ E. coli HCP ELISA Kit offers a wide dynamic range, thereby reducing the number of plates and time required for experiments. The protein standard provided in the kit was used to construct a standard curve spanning from 2.6 to 250 ng/mL. The assay successfully validated the effectiveness of this broad range and demonstrated an exceptionally strong goodness of fit (R2 > 0.99). Disclaimer This product is for research use only and is not intended...