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- Rockland Immunochemicals, Inc,
- AccuSignal™ E. coli HCP ELISA Kit
AccuSignal™ E. coli HCP ELISA Kit
AccuSignal™ E. coli HCP ELISA Kit
Description: The AccuSignal™ E. coli HCP ELISA Kit (KJB-4003) is designed for detecting host cell protein (HCP) contaminants in E. coli samples. It is suitable for ELISA applications with a detection range of 2.6 – 250.0 ng/mL. The kit uses rabbit polyclonal antibodies.
Product Details
Background: The kit's antibodies are tailored to detect HCP contaminants in E. coli cell lysates, specifically from the BL21 strain. It includes a broad coverage antibody for a wide range of proteins.
Components: The kit contains a 96-well strip plate, plate sealer, E. coli-HCP standard, detection antibody, sample buffer, wash buffer, TMB buffer, stop buffer, and protocol.
Application Details
Tested Applications: ELISA
Application Note: This is a sandwich ELISA kit where E. coli proteins are captured and detected using specific antibodies. The chromogenic change is measured at 450 nm, and the HCP concentration is determined using a calibration curve.
Formulation
Physical State: Liquid and Lyophilized
Shipping & Handling
Shipping Condition: Wet Ice
Storage Condition: Store at 2-8°C. Reagents should reach room temperature before use. Reconstituted standard solutions should be aliquoted and stored at -20°C.
Images
The kit provides a wide dynamic range, reducing the number of plates and time required for experiments. The standard curve spans from 2.6 to 250 ng/mL with a strong goodness of fit (R2 > 0.99).
Disclaimer
This product is for research use only and not for therapeutic or diagnostic applications. It is derived from materials of North American origin, inspected and certified to be disease-free. Users must determine the suitability of the materials for commercial use.
Catalog excerpts
Description: AccuSignal™ E. coli HCP ELISA Kit - KJB-4003 Applications: ELISA Host Species: Rabbit Background: The antibodies in the kit have been specifically designed to detect HCP contaminants and to measure analytes from crude cellular harvests to process intermediates. The E. coli ELISA Kit uses purified antibodies generated against a mixture of proteins of mock induced E. coli cell lysate. These antibodies react strongly with E. coli cell lysates from a commonly used E.coli strain, BL21. The key component of this kit is a broad coverage antibody produced against a spectrum of host cell proteins including both low abundancy and high abundancy proteins. Synonyms: host cell proteins, ELISA, process development Host Species: Rabbit Clonality: Polyclonal Detection Kit Type: ELISA Kit Detection Range: 2.6 - 250.0 ng/mL Purity/Specificity: This kit contains: E. coli-HCP antibody coated 96-well strip plate, Plate Sealer, E. coli-HCP standard, E. coli-HCP detection antibody, sample buffer, Wash Buffer, TMB Buffer, Stop Buffer, and protocol. Relevant Links: • KJB-4003 Protocol
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Tested Applications: ELISA Application Note: The AccuSignal™ E. coli HCP ELISA Kit is a sandwich, enzyme-linked immunosorbent assay. The impurities in process-derived samples containing E. coli proteins are captured and detected in a microtiter-type plate by using pre-coated, purified, rabbit polyclonal, E. coli-HCP-specific antibodies. The immunological reaction after the addition of TMB results in a chromogenic change that can be measured photometrically at 450 nm with reference wavelength at 630-650 nm. The O.D. in the wells is directly proportional to the amount of HCP concentration and correlates...
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Order online now! www.rockland.com [email protected] +1 484.791.3823 ELISA Accusignal™ E. coli HCP ELISA Kit and Components for ELISA ELISA The AccuSignal™ E. coli HCP ELISA Kit offers a wide dynamic range, thereby reducing the number of plates and time required for experiments. The protein standard provided in the kit was used to construct a standard curve spanning from 2.6 to 250 ng/mL. The assay successfully validated the effectiveness of this broad range and demonstrated an exceptionally strong goodness of fit (R2 > 0.99). Disclaimer This product is for research use only and is not intended...
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