Description: Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane Background: FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax™ is designed for both Western blotting and enzyme-linked immunosorbent assay (ELISA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate. Synonyms: Peroxidase Substrate, ECL HRP Substrate, Chemiluminescent Femtomax™ Super Sensitive horseradish peroxidase (HRP) Substrate For Microwell And/Or Membrane (2 Component System) Relevant Links: • FemtoMax SDS Suggested Applications: ELISA (Based on references)

Application Note: Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio. FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates...) to minimize background reactivity associated with non-specific...

Order online now! www.rockland.com [email protected] +1 484.791.3823 ELISA The subtests of the Korean version of Consortium to Establish a Registry for Alzheimer’s disease (CERAD-K) and plasma oligomerized beta amyloid (OAβ). (a) Raw scores of the CERAD-K and OAβ concentration. (b) The abnormal CERAD group (below −1.5 standard deviation of age/sex/education adjusting norms) in verbal fluency, naming, word memory/recall, and total scores showed higher OAβ concentration compared with control. (c) Abnormality in naming, word memory/recall/recognition, and total scores are significantly more frequent...

Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane (2 component system) Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate and competitor comparison. GAPDH protein was dotted on a nitrocellulose membrane, load 1000, 250, 62.5, 15.6ngs. The membrane was blocked for one hour at room temperature. Primary antibody: Rb anti-GAPDH diluted 1:2000 and incubated at 4°C overnight. After washing, secondary antibody: Gt anti-Rb IgG-HRP diluted 1:50,000 at room temperature for 2 hours. Detection: Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate or competitor...

Western Blot Western Blot of anti-GST tag antibody. Lane 1: Recombinant GST tagged recombinant protein 5 ug. Lane 2: Recombinant GST tagged recombinant protein 2 ug. Lane 3: Recombinant GST tagged recombinant protein 1 ug. Lane 4: Recombinant GST tagged recombinant protein 500 ng. Lane 5: Recombinant GST tagged recombinant protein 250 ng. Lane 6: Recombinant GST tagged recombinant protein 100 ng. Lane 7: Recombinant GST tagged recombinant protein 50 ng. Primary antibody: anti-GST antibody at 1:1000 for overnight at 4°C. Secondary antibody: donkey secondary antibody at 1:10,000 for 45 min at RT....

• Mollin M et al. Clinical, functional and genetic characterization of 16 patients suffering from chronic granulomatous disease variants - identification of 11 novel mutations in CYBB. Clin Exp Immunol. (2021) • Durbano HW et al. Aberrant BMP2 Signaling in Patients Diagnosed with Osteoporosis. Int J MolSci. (2020) • Zhang L et al. Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation. Histochem Cell Biol. (2020) • Nguyen J et al. A Synthetic Peptide, CK2. 3, Inhibits RANKL-Induced Osteoclastogenesis through BMPRIa...

Order online now! www.rockland.com [email protected] +1 484.791.3823 Beaumel S et al. Identification of NOX2 regions for normal biosynthesis of cytochrome b558 in phagocytes highlighting essential residues for p22phox binding. Biochem J. (2014) Hohne C et al. High mobility group box 1 prolongs inflammation and worsens disease in pneumococcal meningitis. Brain. (2013) Huang BW et al. Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. FASEB J. (2013) Kim MK et al. Differential protective effects of exenatide,...