ANESTHESIA SYSTEM How does it work? Description ANALOG

Rothacher Medical GmbH Spisi 39 CONNECTING DIAGRAM Combi-vet Digiflow or Analog Anesthesia Machine Nose cone Mask System PS-0525-A 02 Oxygen Cylinder with pressure manometer 3 Bar or Wall mount port Aldasorber PS-0584 or PS-0581-00 F/Air Filter Aldasorber PS-0584 or PS-0581-00 F/Air Filter

Rothacher Medical GmbH Spisi 39 ANALOG VERSION Before working please weight the filters, they should not be up to 50 grams more than written on those: The F/AIR Filter 50 g must be exchanged after, approx. 10— 14 hours. Instead of the F/Air Filter, you can also use the Aldasorber 1400 g unit. This Filter must be changed after approx. 40 hours of using.

Verify also the isoflurane level: It should be between the two white stripes: If you need to fill it, please do it in this way: take awy the metal rectangle

The Combi-vet system can be used also with a humidification system mounted at the Dual Diverter. (This device is used for humidification for the lungs of the rodent). At last, fill the oxygen humidificator with distilled water (blue bottle "eau distillee"): Screw open and fill it, then replace Close the "gas dispensers" on the right of the device: Blue bottle "eau distilee" Right valve perpendicular (close) and selector at the left parallel (open) to the tube : Open the oxygen bottle; you do not need to open it completely, just few turns: The regulator should be between 30 (nearly empty) and...

Rothacher Medical GmbH Turn the scroll (white knob) Disinfect green box, table, very gently and set to the column at 0.8 mask... with ethanol 70% Then open the isoflurane dial at the vaporizer Push on the white button then turn until you are on 0: Verify that on the left side of the selector is open: Let the one with the white stripe close Then you can move the wheel without pushing the button: At 4.5 to 5 % it is induction Of anesthesia in the green box

A Rothacher Medical GmbH Wait for mice anesthesia, you will see by shaking softly the box if it is asleep: It should fall on the side and if it is not completely anesthetized, it should try to recover sternal recumbency. If it is asleep, it will not move. Then take the mouse out, put it on the table and verify that it is sleeping well with toe clipping: Softly press the toes between two fingers and if the mouse does not take off its leg, it is asleep. Change the selector: close the left and Moreover, lower isoflurane open the right one, with the white stripe: to 1.5 to 2 %:

A Rothacher Medical GmbH TAKE CARE THAT MICE IS STILL BREATHING QUIETLY (AROUND 170 PER MINUTE, ~ 3 / SECOND) IF BREATHING IS LABORING AND AROUND 80 PER MINUTE (~ 1 / SECOND), MOUSE IS ON ISOFLURANE OVERDOSE, STOP IT IF OPERATION IS ENDED OR LOWER IT TO 1 %. ANOTHER IMPORTANT POINT TO CONSIDER: ISOFLURANE DOES NOT HAVE ANY ANALGESIC PROPERTY, SO FOR AN OPERATION (OBLIGATORY) OR A MANIPULATION POTENTIALLY PAINFUL, GIVE ANALGESICS. YOU HAVE SEVERAL OPTIONS: OPIOIDS FOR PAINFUL OPERATIONS CARPROFEN (NSAID) FOR LESS PAINFUL MANIPULATIONS THEN ACETAMINOPHEN IF PAIN IS MODERATED OR OPIOIDS IF PAIN...