A Rothacher Medical GmbH

CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch

CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch FIGURE INDEX Figure 1 "Basic Anesthesia System with Mouse Nosecone/Face Mask" Page 1 Figure 2 "Dual Diverter Manifold with Humidifier" Page 7 Figure 3: "Multiple Delivery System for 6 Animals" Page 7 Figure 4: "Nosecone/Face Mask (above) and Induction Box (below)" Page 8 Figure 5: "Double Scavenging System with 2 Activated Charcoal Canisters" Page 8 Figure 6: "Activated Charcoal Canister" Page 9 Figure 7: "Filling a Key-Fill Vaporizer" Page 10 Figure 8: "Filling the...

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch ANESTHESIA SYSTEMS CV 30-301 Series The Combi-vet Anesthesia system has been especially designed for inhalation of Anesthetic agents to laboratory animals. It is a small weight, compact and robust device. It can be used either on a rail mount system or as a standalone unit on a table. As this device is transportable, it can be moved from one place to another in no time and can be immediately operational. The system is available in different configurations...

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch 1.1 CONNECTING DIAGRAM Combi-vet Digiflow or Analog Anesthesia Machine 02 Concentrator 02 Oxygen Cylinder with pressure manometer or Wall mount port Aldasorber PS-0584 or PS-0581-00 F/Air Filter

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch Check the contents of the shipment for completeness, packing list to hand, and visually inspect the instrument as soon you take it out of the packaging. Use the Check List supplied. If the instrument is damaged or after having tested it, fails to meet rated performances, notify the carrier and our company immediately. Dispose of packaging properly, according to existing and applicable waste management rules and regulations. 2.2 Notes on the Instruction...

Rothacher Medical GmbH Spisi 39 CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch The manifold outlet on the left is disconnected and the right outlet is connected to the humidifier 4. If the Multiple Delivery System option is present in the system, connect the output of the vaporizer to the input of the multiple delivery system Refer to the specific section of the manual for details on use of the Multiple Delivery System).

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch 5. Connect the activated charcoal canister (or the input of the active scavenging system, if present) to the exhalation side of the nosecone/facemask or to the lower port (i.e. the outlet) of the induction box.

Rothacher Medical GmbH Spisi 39 CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch The activated charcoal filters absorb the anesthetic agent. Their weight will increase with use and will need to be changed when the weight is increased by 50 grams (after 14-15 hours of use). a. Check all connections and parts before use, make sure there is no leakage The use of scavenging canisters is compulsory, unless the anesthesia system is connected to a flow hood or another scavenging system is in use 2.5 Important Safety Consideration...

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch Before operating the machine, read all manuals (including the ones for Vaporizer and Flowmeter) included. The following is an example procedure to help the user familiarize with the equipment. It assumes that the a mouse is to be anesthetized using a Gas Anesthesia system, which includes: • Tec3 Key-fill Isoflurane Vaporizer • dual diverter manifold with humidifier • nose-cone/face mask and induction box • 2 activated charcoal canisters as scavengers...

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch IMPORTANT! If any spillage accidentally occurs, immediately open all windows and do not use the room until the environment is safe again. 5. Fill-in the humidifier system, which is connected to one of the outlets of the dual diverter manifold 6. Make sure that the output of the Dual Diverter that connects to the induction box is open and that the one that connects to the Nose-cone/facemask is closed. 7. Turn on the vaporizer to the desired concentration...

Rothacher Medical GmbH CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch To turn the vaporizer dial, press the button and turn until the zero value is reached. At that point, the dial can be turned without any further pressure of the button. 8. Quickly put the mouse in the induction camber and close the box properly. Wait for the mouse to be fully anesthetized (this will occur quite quickly) and then gently shake the box to check if it is asleep. If a mouse is asleep, it will fall on the side, without trying to recover...

CH-1714 Heitenried, Switzerland Tel: +413137112 81 Fax: +413137153 35 eMail: [email protected] www.rothacher-medical.ch 12. When finished, turn off the vaporizer (setting the dial to off); the animal will receive pure oxygen and it could help to wake up and recover more quickly 13. Turn off the oxygen source and turn off the flow-meter to zero LPM 14. Disinfect the induction box, the operation table, etc. with 70% Ethanol and rinse with water. All of the Multiple Delivery Systems (for 3,4 and 6 animals) must be used with an anesthesia machine that has a 0-12 liter per minute Oxygen flow-meter...