%5 Sheep Blood Agar
2Pages

RTA.KK.126 Revision Date/Revision Number:-/0 Issue Date: 01.11.2014 INTENDED USE: 5% Sheep Blood Agar is a highly nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from clinical specimens. PRINCIPLE AND INTERPRETATION: 5% Sheep Blood derives its superior growth-supporting properties from the combination of two peptones, and yeast extract as a supplier of the B complex vitamins. Corn starch is included to absorb toxic by-products contained in the specimen and serves as an energy source for organisms possessing alphaamylases. Sheep blood allows detection of hemolytic reactions and supplies the X factor (heme) necessary for the growth of many pathogenic species. On this medium, colonies tend to be larger and growth is more luxuriant than on media. Casein enzymic hydrolysate and yeast extract provide nitrogen, carbon, amino acids and vitamins. Peptic digest of animal tissue is the nitrogen source. Sodium chloride maintains the osmotic balance. COMPOSITION: Ingredients ***Formula adjusted, standardized to suit performance parameters pH: 7,4 ± 0,2 PRECAUTIONS: For professional use only. Do not use plates if they show evidence of microbial contamination, discoloration, drying, cracking or other signs of deterioration. TEST PROCEDURE: 1. Inoculate representative samples with the cultures diluted to contain 50-100 CFU per 0,1 mL. a. Add 0,1 mL of the appropriate dilution to each plate and spread-inoculate using a sterile glass spreader. b. Incubate the Escherichia, Shigella and Staphylococcus strains at 35 ± 2°C in an aerobic atmosphere and the Streptococcus at 35 ± 2°C in an aerobic atmosphere supplemented with 3-5% carbon dioxide. 2. Examine plates after 24-48 h for amount of growth, colony size and hemolytic reactions. QUALITY CONTROL: l.Sterility Control: Incubation 48 hours at 30-35°C and 72 hours at 20-25°C: NO GROWTH 2.Phsical/Chemical Control pH: 7,4 ± 0,2 Apperance: Red 3.Microbiological Control: Incubation at 30-35 °C during 24-48 h STORAGE CONDITIONS AND SHELF LIFE: Store the prepared medium at 2 - 12°C. Use before expiry date on the label. Do not use beyond stated expiry date. RTA LABORATUVARLARI BlYOLOJlK URUNLER lLAQ VE MARINE SAN. TlC. A.§. Plastikfiler Organize Sanayi Bolgesi, Cumhuriyet Cad. No:3 41400 Gebze/Kocaeli/TURKlYE Phone: +90 262 648 53 00 Fax: +90 262 751 06 77 E-mail: rta@rtalabs.com.tr Web: www.rtalabs.com.tr

Technical Data Sheet Sayfa 2 / 2 RTA.KK.126 Revision Date/Revision Number:-/0 Issue Date: 01.11.2014 DISPOSAL: Incubated prepared medium may contain active bacteria and micro-organisms. Do not open infected medium. Infected plate should be autoclaved, incinerated or opened and soaked in a chlorine-based disinfectant (liquid bleach) for 20 minutes prior to disposal. PACKAGING: Katalog Number: 02002 Packaging: Single wrap Content: 10 plates/each package REFERENCES: 1. Ellner, P.D., C.J. Stoessel, E. Drakeford, and F. Vasi. 1966. A new culture medium for medical bacteriology. Am. J. Clin....