Technical Data Sheet Sayfa 1 / 2 RTA.KK.123 Revision Date/Revision Number:-/0 Issue Date: 01.11.2014 INTENDED USE: A selective and diagnostic medium for the isolation and enumeration of Staphylococcus aureus in foods PRINCIPLE AND INTERPRETATION: Baird-Parker added sodium pyruvate, to protect damaged cells and aid their recovery2 and egg yolk emulsion as a diagnostic agent. The selective agents glycine, lithium and tellurite have been carefully balanced to suppress the growth of most bacteria present in foods, without inhibiting Staphylococcus aureus. Egg yolk emulsion makes the medium yellow and opaque. Staphylococcus aureus reduces tellurite to form grey-black shiny colonies and then produces clear zones around the colonies by proteolytic action. This clear zone with typical grey-black colony is diagnostic for Staphylococcus aureus. On further incubation, most strains of Staphylococcus aureus form opaque haloes around the colonies. This is probably due to the action of a lipase. Not all strains of Staphylococcus aureus produce both reactions. Some strains of Staphylococcus saprophyticus produce both clear zones and opaque haloes but experienced workers can distinguish these from Staphylococcus aureus by the longer incubation time required5. COMPOSITION: ***Formula adjusted, standardized to suit performance parameters pH: 6,8 ± 0,2 PRECAUTIONS: For professional use only. Do not use plates if they show evidence of microbial contamination, discoloration, drying, cracking or other signs of deterioration. TEST PROCEDURE: 1-Dry the surface of agar plates for a minimal period of time prior to use. 2-With a glass spatula, spread 0,1ml aliquots of food dilutions made up in Buffered Peptone Water on the agar surface until it is dry. Up to 0,5ml may be used on larger dishes. 3-Incubate the inverted dishes at 35°C. Examine after 24 hours and look for typical colonies of Staphylococcus aureus. Re-incubate negative cultures for a further 24 hours. QUALITY CONTROL: l.Sterility Control: Incubation 48 hours at 30-35°C and 72 hours at 20-25°C: NO GROWTH 2.Phsical/Chemical Control pH: 6,8 ± 0,2 Apperance: Amber3.Microbiological Control: Incubation at 35+2 °C during 24-48 h RTA LABORATUVARLARI BlYOLOJlK URUNLER lLAQ VE MARINE SAN. TlC. A.§. Plastikfiler Organize Sanayi Bolgesi, Cumhuriyet Cad. No:3 41400 Gebze/Kocaeli/TURKlYE Phone: +90 262 648 53 00 Fax: +90 262 751 06 77 E-mail: [email protected] Web: www.rtalabs.com.tr

Technical Data Sheet Sayfa 2 / 2 RTA.KK.123 Revision Date/Revision Number:-/0 Issue Date: 01.11.2014 STORAGE CONDITIONS AND SHELF LIFE: Store the prepared medium at 2 - 12°C. Use before expiry date on the label. Do not use beyond stated expiry date. DISPOSAL: Incubated prepared medium may contain active bacteria and micro-organisms. Do not open infected medium. Infected plate should be autoclaved, incinerated or opened and soaked in a chlorine-based disinfectant (liquid bleach) for 20 minutes prior to disposal. PACKAGING: Katalog Number: 02001 Packaging: Single wrap Content: 10 plates/each package...