Technical Data Sheet Sayfa 1 / 2 RTA.KK.103 Revision Date/Revision Number:-/0 Issue Date: 01.11.2014 INTENDED USE: Cetrimide Agar is used for the selective isolation and presumptive identification of Pseudomonas aeruginosa from clinical and nonclinical specimens. PRINCIPLE AND INTERPRETATION: Cetrimide is a quarternary ammonium compound with bactericidal activity against a broad range of Gram-positive organisms and some Gram-negative organisms. Pseudomonas aeruginosa produces a number of water soluble iron chelators, including the yellow-green or yellow-brown fluorescent pyoverdin. When pyoverdin combines with the blue water-soluble pyocyanin, the bright green colour characteristic of Pseudomonas aeruginosa is created. The addition of magnesium chloride and potassium sulphate enhances the production of these chelators. COMPOSITION: LIMITATIONS OF THE PROCEDURE: This prepared plated medium is intended for primary isolation. Some diagnostic tests may be performed with the primary plate. However, a pure culture is recommended for biochemical tests and serological procedures. Consult appropriate texts for further information. RTA LABORATUVARLARI BlYOLOJlK URUNLER lLAQ VE MARINE SAN. TIC. A.§. Plastikfiler Organize Sanayi Bolgesi, Cumhuriyet Cad. No:3 41400 Gebze/Kocaeli/TURKIYE Phone: +90 262 648 53 00 Fax: +90 262 751 06 77 E-mail: [email protected] Web: www.rtalabs.com.tr

Technical Data Sheet Sayfa 2 / 2 RTA.KK.103 Revision Date/Revision Number:-/0 Issue Date: 01.11.2014 A single medium is rarely adequate for detecting all organisms of potential significance in a specimen. The agents in selective media may inhibit some strains of the desired species or permit growth of a species they were designed to inhibit, especially if the species is present in large numbers in the specimen. Cultures of specimens grown on selective media should, therefore, also be cultured on nonselective media to obtain additional information and help ensure recovery of potential pathogens....