R821 Fiber photometry Brochure

Product catalog summary

Introduction
The Multichannel Fiber Photometry System (R811/R821) is engineered for monitoring fluorescence intensity changes in neurons, indicative of neuronal activity. It integrates optogenetics for concurrent recording and stimulation, facilitating long-term neuronal monitoring in freely moving animals to study neural circuits and behavior.

Product Features
The system includes three excitation light sources (410nm, 470nm, 560nm) and supports up to 9 channels for multi-animal or multi-location experiments. It features dual sensitive detectors for independent detection, reducing interference and improving signal accuracy. The system offers professional acquisition and analysis software, eliminating the need for MATLAB programming, and supports various acquisition modes such as continuous, interval, trigger-based, delayed, and timed. Real-time signal changes can be monitored via Live DeltaF/F acquisition display, and output signal parameters are customizable for closed-cycle control.

Product Parameters
Key parameters include adjustable excitation light power (0µW to ≥100µW), a maximum frame rate of 250fps, and a behavior camera resolution of 1920x1080 at 30fps or 1280x720 at 60fps. The system provides manual, automatic, and ROI marking capabilities, and an output frequency range of 0-500Hz with adjustable pulse width and duration.

Hardware Functions
The system includes 4 input ports for external TTL signal triggers and marking, and 4 output ports for triggering external equipment. It supports various optical fiber sizes with a lightweight focusing interface and includes a port for optogenetics connection.

Software Functions
The software offers stable data acquisition and processing, with features like data clipping, bleaching correction, smoothing, movement correction, event heat map, peak statistics, and area under curve. It allows one-click generation of peri-event heat maps, supports data group comparison, and flexible event handling. Results can be exported in formats such as DeltaF/F, Z-score, and AUC.

Standard Configuration
The package includes a host, power cord, USB cables, USB expansion interface, and software U disk. The pre-installed software runs on a computer with specifications: I5-10500H/16G/500G/WIN10. It also includes low autofluorescence fiber-optic patch cords, black ceramic sleeves, a behavior camera and bracket, a photobleaching device, and optional accessories like a fiber-optic cannula and laser power meter.

Specifications
Manual marking offers 10 levels, automatic marking 4 levels, and ROI marking 9 levels. The output frequency is adjustable from 0-500Hz, with customizable pulse width and duration.

Components
The system comprises the Fiber Photometry Main Device, Behavior Camera bracket, Photobleaching device, U disk, Computer, Optical fiber, Fiber Cannula sleeves, and Behavior Camera.

Optional Accessories
Optional accessories include a Fiber-optic Cannula, Branching Fiber-optic Patch Cord, Laser power meter, and Ceramic Ferrule Holder.

Additional Information
The system can record animal behavior video and identify animal tracks using USB3.0, 3M.

Catalog excerpts

Standard Conﬁguration Product Features CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY Three kinds of excitation light sources, that is 410nm、470nm and 560nm, are respectively used for excitation Fiber Photometry Main Device Optical ﬁber Fiber Cannula sleeves Behavior Camera Supports multiple acquisition modes including continuous acquisition, interval acquisition, acquisition upon Behavior Camera bracket Live display of DeltaF/F acquisition to check scale of signal changes during acquisition; of reference, green ﬂuorescence and red ﬂuorescence; Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain locations; Dual highly sensitive detectors enabling independent and sequential detection to avoid interference of ﬂuorescence excitation and detection, acquiring more accurate signal; Professional acquisition and analysis softwares are ﬂexible and easy to operate with data processing functions available. No matlab programming is required; trigger, delayed acquisition and timing acquisition; Customized adjustment of output signal parameter, easily trigger and control external excitation equipment Photobleaching device Includes: Host, power cord, 3 USB cables, USB expansion interface, software U disk Includes pre-installed software, I5-10500H/16G/ 500G/WIN10(1920*1080) Low Autoﬂuorescence Fiber-optic Patch Cords 200um/ 0.37NA/2m,Ф1.25mm or Ф2.5mm Black Ceramic Sleeves, Φ1.25mm or Φ2.5mm Record video of animal behavior and identify animal tracks,USB3.0,3M Adjustable height range0.8-1.5m,Rotation Angle 360° FC/PC Patch Cord photobleaching machine(R810-1) Software key(Not for analysis function) to achieve closed-cycle control of excitation and recording; Compatible with optogenetics for recording and stimulation at the same site. Product Parameters CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY Wavelength of excitation light Power Number of channels Min 0µW, Max≥100µW, adjustable with an accuracy of 0.1µW 9 Frame rate of ﬂuorescent sampling Digital signal interface Signal output Output frequency 0-500Hz, adjustable output pulse width and duration Marking Behavior camera Manual marking (10), automatic marking (4), ROI marking (9) 1920*1080(30fps) 1280*720(60fps) Switchable among multiple frame rates of resolution Optional Accessories：Fiber-optic Cannula, Branching Fiber-optic Patch Cord, Laser power meter and Ceramic Ferrule Holder etc. Client List CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY Multichannel Fiber Photometry System (Compati

Open the catalog to page 1

CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY The ﬁber photometry system records changes in the ﬂuorescence intensity of neurons in a speciﬁc brain area to reﬂect neuronal population activity. In the study of neural circuits, the ﬁber photometry system can perform Software functions CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY Professional acquisition and analysis softwares CONTRIBUTE WISDOM AND STRENGTH TO THE IMPROVEMENT OF QUALITY long-term stable monitoring of the neurons of freely moving animals, and explore the correlation between neural activity and animal...

Open the catalog to page 2