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RWD From Function to Selection About Cryostat Machine

RWD From Function to Selection About Cryostat Machine
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RWD From Function to Selection About Cryostat Machine - 1

From Function to Selection: Everything You Need to Know About Cryostat Machine What Is Cryostat? Cryostat is a sectioning instrument which maintains low-temperature conditions for the tissues to reach a certain level of hardness by rapid freezing. Then, it is used for cutting the sample in slides within a certain thickness range, preparing the tissue samples for other relevant operations. It is applicable to the sectioning of fresh tissue samples, including animals, plants and human body tissues. The cryostat temperature range: chamber temperature: -18~20 ℃ ; specimen head temperature: -15~-20℃. Breast adipose tissue is the hardest one to handle among all the sample sectioning. The chamber temperature has to be set at its minimum and the specimen head temperature has to be adjusted to -50℃. The section thickness could be set at 10μm -15μm. Frozen section thickness range: 5μm ~20μm. 6-8μm for clinical applications. 5μm works best for lymph nodes as their cells are denser. For scientific research, section thickness is commonly set at 8μm, -10μm or 20μm. Cryostat Working Principle After setting up the optimal temperature for sectioning, a tissue sample of standard size should be embedded with the optimal cutting compound on the sample plate. Then, It should be embedded with the optimal cutting compound on the sample plate. Upon the completion of embedding, mount the sample on the specimen head and use the sample injection method to obtain frozen sections. Application Cryostats are widely used in domains such as histology, pathology, cytology, neurology and so on, including immunofluorescence technique, immunohistochemistry technique, in situ hybridization technique; non-enzyme substance identification and research; neuropathology research; spatial transcriptomics sect

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Types Of Cryostat The most common types of frozen sectioning machines are semi-automated and fully automated cryostats. The semi-automated cryostat possesses a cryochamber and a specimen head for separate freezing. Both are engineered with motorized sample injection and UVC disinfection. For seasoned technicians, a semi-automated cryostat is the first choice for higher sectioning efficiency. The fully automated cryostat does not only possess the features of the semi-automated one, it also has a cut window function that allows the setting of the size of the section to prevent any subsequent...

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Cryostat Parts And Functions A cryostat machine usually encompasses the cooling, sectioning, safety and operation system. cryostat structure Refrigeration System Cryochamber and specimen head carry out cooling separately. For the cryochamber, a compressor is used for cooling. As temperature difference arises during sectioning, the compressor provides double compensation for temperature loss to maintain the cryochamber temperature and ensure the quality of the section results. As for the specimen head, a low-pressure refrigeration system and semi-conducting elements are adopted for cooling,...

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effectively kills infectious tissues, bacteria and viruses. The waste liquid management system sends out a warning when the current waste bottle is not in a position or the waste liquid overflows. This prevents the waste liquid from flowing out and causing contamination to the ground so as to protect the laboratory staff and uplift the quality control and management standard of the laboratory. Operation System Specimen head orientation is visible during adjustment that offers an effective solution to the time-consuming and energy-draining process of adjusting the specimen head. The...

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For Amateurs (5) Choose a well-known brand because of its cutting-edges of quality and stability. RWD cryostat displays a short service response time and offers a comprehensive user service network. Its user landscape covers over 100 regions around the world. Meanwhile, choose the most suitable brand and model based on the actual needs of the laboratory. Don’t blindly pursue high-end instruments or inexpensive equipment. (6) If conditions allow, the best practice is to invite a professional technician to test the product on spot. This could greatly reduce the chances of buying substandard...

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