Used the Laser Speckle Imaging System to monitor the blood perfusion for Hind Limb Ischemic (HLI) The rodent limb ischemia preparation is a wild used model of peripheral arterial disease (PAD). The model uses a https://www.rwdstco.com/product-item/laser-speckle-imaging-system/ to real-time measure blood perfusion (PU) in limbs, and we can get imaging of blood vascular to distribute. When we draw the ROIs (region of interest), the software enables the measurement of the blood flow. As we can see, the difference in PU between the ischemic versus and non-ischemic limb (Fig1). And we can do some research about the “Reperfusions” which can measure the function of drugs in the PAD. And if you want to have a free demo of the laser speckle,http://online.rwdstco.com/laserspeckleimage . Equipment recommendations The Laser Speckle Contrast Imaging System (RFLSI Ⅲ ) offers the highest spatial resolution (2048 × 2048) and can be used to assess reperfusion in the pads of the feet(https://pubmed.ncbi.nlm.nih.gov/32647036/). RFLSI recording the process of reperfusions and vessel formation

Fig 1. Right limb: ischemic versus, left limb: non-ischemic limb. Reference: https://pubmed.ncbi.nlm.nih.gov/32647036/