Fluorescent Detection Reagent
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SAS™ Loopamp® Fluorescent Detection Reagent • Amplification results can be observed visually The Loopamp® Fluorescent Detection Reagent is added to the reaction tube during the reaction setup. The LAMP amplification is then performed at a constant temperature (60-65°C) in a heat block or a water bath for the reaction time required by a specific primer set. The enzyme is Visual endpoint detection inactivated by heating the tubes at 80-95°C for 2 minutes. Presence of fluorescence under a UV light (254-366 nm) For use with any indicates a positive result. Loopamp® detection or amplification kit No special detection equipment required • Turbidity detection will not be affected The presence of this reagent will not affect the result by real-time turbidity detection (standard detection method) using a Loopamp® Realtime Turbidimeter. • No extra effort to confirm the amplification Amplification results can be visually observed through the presence of fluorescence. Because confirmation of results with electrophoresis is not required, the possibility of crosscontamination frequently associated with DNA/RNA amplification technologies is great

SAS™ Loopamp® Fluorescent Detection Reagent The Principle The LAMP (Loop-mediated Isothermal Amplification) method can produce a large amount of DNA/RNA amplification product as well as its by-product, magnesium pyrophosphate. The detecting reagent calcein is initially bound with a quenching manganese ion. When the amplification reaction proceeds, the manganese ion binds with higher affinity to the generated pyrophosphate, which results in the emission of fluorescence from the calcein. The free calcein then binds with magnesium ions in the reaction mixture, which strengthens the...