SAS™ Molecular Tests Verotoxin-producing Escherichia coli Detection Kit • Rapid and specific detection of Verotoxin-producing Escherchia coli in foods The Verotoxin-producing Escherichia coli Detection Kit Highly sensitive and uses the Verotoxin (VT, also known as Shiga toxin or Shiga specific like toxin) genes as the target sequences. By using four primers to recognize six distinct regions in the genes, Amplification and this test is highly specific. Due to the high amplification detection in 60 minutes efficiency, test results are achieved within one hour, in a single tube following sample enrichment. Automated real-time • Amplification and detection in a single tube detection with real-time results High volume throughput With Realtime Turbidimeters, amplification and capacity - up to 96 samples real-time detection are achieved in one step. per hour, including controls Since electrophoresis is not required, the entire process is completed within one reaction tube, which greatly reduces the possibility of crosscontamination frequently associated with nucleic acid amplification technologies.

SAS™ Molecular Tests Verotoxin-producing Escherichia coli Detection Kit Procedure Preparing the master mix for 1 test Sample enrichment Heat at 95°C for 5 minutes, then centrifuge for 1 minute Dispense 20 µL of Master Mix into each reaction tube Reaction tubes Place tubes in turbidimeter at 65°C and start program Read real-time results Kit Contents Extraction Solution for Foods Reaction Mix VT Bst DNA Polymerase Control DNA VT 1.8 mL x 2 tubes 0.5 mL x 2 tubes 60 µL x 1 tube 0.1 mL x 1 tube Ordering Information Description SAS™ Verotoxin-producing Escherichia coli Detection Kit SAS™ Reaction...