Canine Parvovirus Antigen Test Kit Disease Background Information Canine parvovirus (CPV) disease is currently the most common infectious disorder of dogs in the United States. CPV is a highly contagious disease characterized by diarrhea that is often bloody and is caused by a pathogen called canine parvovirus, Type 2 (CPV-2). The most common form of the disease is the intestinal form known as enteritis. CPV-2 enteritis is characterized by vomiting, diarrhea, dehydration, dark or blood feces, and in severe cases, fever and lowered white blood cell counts. Current vaccinations have helped to control the spread of this disease but despite being vaccinated, some dogs still contract CPV and die from it. The majority of cases are seen in dogs less than 6 months of age, with the most severe cases seen in puppies younger than 12 weeks of age.1 Test Principle This test is a rapid (10 minute) assay based on the detection of canine parvovirus specific antigens in canine fecal samples. The assay uses sensitized gold particles to bind up this antigen and deposit at the test line. The accumulation of this gold particle/antigen complex at the test line results in a band (line) that can be seen visually. A second control line indicates that the test has been performed correctly. Five Test Kit Fifteen Test Kit Thirty Test Kit Test Devices: 5, 15 or 30 individually packaged test devices. Sample Dilution Buffer: 5, 15 or 30 tubes containing 1 ml of sample dilution buffer with sodium azide as a preservative. Transfer Pipettes: 5, 15 or 30 Collection Swabs: 5, 15 or 30 Instruction for use: 1

Use the kit before the expiration date indicated on the kit box. Store the kit between 2 ºC and 7 ºC (36 ºF to 45 ºF). Allow the kit to come to room temperature 15 ºC to 25 ºC (59 ºF to 77 ºF) before using. Open the pouch containing the device immediately before use. Run the test on a flat, horizontal surface. Hold the sample pipette vertically when adding reagents to the sample window of the test device. Do not touch the white membrane surface at any time. This could tear the membrane resulting in an inaccurate test. Add only the correct amount of diluted fecal sample to the device. Dilution...

Positive: Any visible line in the cassette window at both the test and the control line area indicates that Canine Parvovirus specific antigens are present in the sample. Negative: A line will be visible in the cassette window only in the control line area. This indicates that Canine Parvovirus specific antigens are not present in the sample or are present at a concentration level below the detection limit of this assay. Invalid Test: If no lines are visible in the cassette window OR if only the test line is visible the test is invalid. Any invalid test should be repeated. Positive Negative Invalid Invalid...