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Cell and Tissue Culture

Cell and Tissue Culture
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Cell and Tissue Culture

Product catalog summary
Tissue Culture Products Overview
Sarstedt provides a comprehensive range of certified tissue culture products that adhere to strict purity standards, crucial for reliable in vitro applications. These products are utilized in fields such as basic research, biotechnology, clinical research, toxicity testing, and industrial applications like monoclonal antibody production. The product lineup includes TC Flasks, TC Dishes, 96/24/6 Well TC Plates, Cell Scrapers, and Filtration Units, all manufactured under stringent conditions to ensure sterility, non-pyrogenicity, and non-cytotoxicity.
Innovative Features of Tissue Culture Flasks
The flasks are equipped with an anti-drip lid and a vented cap featuring a hydrophobic filter membrane for consistent gas exchange. The quick-release cap system facilitates easy handling and ensures proper ventilation with a distinct click. Additionally, the flasks have a special pouring channel for efficient media removal and an anti-tip skirt to minimize spillage risk. The Cell+ growth surface supports various adherent cells, eliminating the need for additional coatings.
Tissue Culture Dishes and Plates
SUREGrip dishes are designed for safe handling with a raised ring for secure grip and stackability. They are available in standard, grid, suspension, and Cell+ surfaces. Tissue culture plates offer three growth surface options and are designed to minimize contamination risk with recessed wells and anti-condensation lids.
Cell Scrapers and Coverslips
Cell scrapers feature a flexible blade for efficient cell recovery, suitable for various culture containers. Coverslips are highly transparent and suitable for microscopy, with surface treatment for easy handling.
Roller Bottles
These bottles are made from durable material and are ideal for large-scale production of biological substances. They offer advantages like an enlarged growth surface and steady medium mixing, with features for safe microscopic control and space-saving incubation.
Filtration Units
Filtration units are non-cytotoxic, offer high flow rates, and are biocompatible. They feature low protein adsorption and economical use due to integral prefilters.
Overview of Filtration Products
The document details various sterile filtration units and related products designed for the filtration of aqueous solutions, cell culture research, microbiology, and cryogenic storage. It highlights the specifications, applications, and benefits of each product line, including Filtropur, CryoPure, and serological pipettes.
Filtropur Filtration Units
  • Types: Filtropur S, Filtropur S plus, and Filtropur L are sterile pressure filtration units.
  • Materials: Utilize biocompatible materials like Cellulose Acetate (CA) and Glassfiber (GF) prefilters to prevent contamination.
  • Applications: Suitable for volumes from milliliters to 10 liters, ideal for solutions difficult to filter, such as serum and culture media.
  • Efficiency: Integral GF prefilter doubles filtration volume, reducing costs and environmental impact.
Filtropur V and BT Vacuum Filtration Units
  • Features: High-flow PES membranes for high throughput and low protein binding.
  • Applications: Ideal for cold sterilization of cell culture media and protein solutions.
  • Specifications: Available in various pore sizes (0.1 µm, 0.22 µm, 0.45 µm) and volumes (250 ml to 1,000 ml).
CryoPure Storage System
  • Quality Assurance: Certified as non-pyrogenic, non-mutagenic, non-cytotoxic, sterile, and CE/IVD-compliant.
  • Design: Available in volumes from 1.2 ml to 5.0 ml with color-coded caps for easy identification.
  • Storage Options: Compatible with various cryo storage boxes, autoclavable, and designed for low-temperature storage.
Serological Pipettes
  • Features: Non-pyrogenic, individually wrapped, and sterile with easy size identification through international color coding.
  • Design Improvements: Enhanced fit in pipette aids to minimize movement and prevent leaks.
Additional Products
  • flexiPERM® and quadriPERM®: Tissue culture dishes and inserts for parallel analyses and cell cultivation.
Product Overview
flexiPERM® is a reusable silicone insert designed to subdivide slides or tissue culture dishes (Ø 50 mm) into smaller cultivation units. It is autoclavable and adheres to various plain surfaces such as glass, foil, and plastic.
Product Specifications
  • flexiPERM® micro: 12 cultivation units, packaging of 5.
  • flexiPERM® slide: 8 cultivation units, packaging of 5.
  • flexiPERM® disc: 4 cultivation units, packaging of 5.
  • flexiPERM® conA: 1 cultivation unit, packaging of 5.
  • flexiPERM® conB: 1 cultivation unit, packaging of 5.
Contact Information
SARSTEDT AG & Co., P.O. Box 12 20, D-51582 Nümbrecht, Phone: +49 2293 3050, Fax: +49 2293 305-122, Email: [email protected], Website: www.sarstedt.com
Additional Notes
Technical modifications reserved. For more information, please call or visit the website.
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Catalog excerpts

Cell and Tissue Culture-1

Cell and Tissue Culture Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling

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Cell and Tissue Culture-2

Contents Tissue Culture Flasks page 4-7 Tissue Culture Dishes page 8 Tissue Culture Plates page 9 Cell Scrapers/Coverslips page 10 Roller Bottles page 11 Filtration Units page 12-14 Tubes page 15 CryoPure page 16-17 Serological Pipettes/Sarpettes page 18 NEW flexiPERM® / quadriPERM® 2 page 19

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Cell and Tissue Culture-3

Tissue Culture Products Come Grow With Us Sarstedt Certified Tissue Culture Products In the field of cell and tissue culture, compliance with strict purity standards is essential to achieving reliability and consistency for in vitro applications. Cell and tissue cultures are now being used in fields other than basic research, applied biotechnology and clinical research. Cell cultures are also increasingly used in toxicity tests, for quality control of chemical and biological products as well as for industrial applications, e.g. manufacturing monoclonal antibodies etc. Sarstedt produces sterile...

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Cell and Tissue Culture-4

Tissue Culture Flasks The Tissue Culture Flask concept with the advanced Capping System Utilize the 2-position Quick-Release cap for rapid and easy handling Leave it to others to waste time and effort removing and replacing caps... Quick-Release cap with open/close indicator • Maximum 1/3 turn for ergonomic opening/closing of the flask • Arrows on the quick-release cap serve as open/closed indicator • Additional visual control of the closed flask: arrows point left to right Open Closed Two-position Screw Cap with positive venting mechanism Lock the cap in the defined vented position by a simple...

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Cell and Tissue Culture-5

Tissue Culture Flasks The Tissue Culture Flask concept that simplifies your daily routine The Colour Code that offers maximum flexibility Depending on the cell’s affinity to the growth surface and your requirements, Sarstedt offers three growth surface options. Adherent cells are easily cultivated in Tissue Culture Flasks with traditional Tissue Culture surface; coded with red or black quick-release cap. Primary, established epithelial and other fastidious cell lines that are difficult to grow on traditional surfaces may grow better on the growth surface offered by Cell+; coded with a yellow...

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Cell and Tissue Culture-6

Tissue Culture Flasks The Tissue Culture Flask concept that simplifies your daily routine Cell+ growth surface supports the cultivation of various adherent cells Cell+, a Sarstedt product, is a positively charged, growth supporting surface, which is particularly suitable for the cultivation of superior cells. By imitation of amino-functional groups of proteins the Cell+ surface allows binding and growth of a large spectrum of cells. The Cell+ surface specifically supports the cultivation of various adherent • primary cultures • continuous cell lines • cells which previously needed a coated surface...

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Cell and Tissue Culture-7

Tissue Culture Flasks The Tissue Culture Flask concept – that simplifies your daily routine Ordering details – Sarstedt colour code system Cell+ tissue culture flasks, with yellow cap, vented or non vented Plug Seal Cap non vented vented Order No. Growth Area Volume Neck Shape Sales Unit bag/case 83.1810.300 83.1810.302 50 ml 50 ml 20 /300 20 /300 83.1813.300 83.1813.302 75 cm2 75 cm2 250 ml 250 ml 5 / 100 5 / 100 83.1812.300 83.1812.302 Cell+ 25 cm2 25 cm2 175 cm2 175 cm2 650 ml 650 ml 5 /40 5 /40 Growth Area Volume Tissue culture flasks with red cap, vented or non vented Plug Seal Cap non vented...

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Cell and Tissue Culture-8

Tissue Culture Dishes Facilitated Handling SUREGrip Dishes for safe and convenient handling • Produced from high-quality polystyrene • A depression in the lid and base makes the dish stackable • Cell+ surface for primary cultures and other superior cell lines • Sterilized by gamma radiation • Special versions with a hydrophobic surface for suspension and hybridoma cultures • Newly designed 35/60/100 mm dishes with SUREGrip Größe 35 x 10 SUREGrip 60 x 15 SUREGrip 100 x 20 SUREGrip 150 x 20 Standard X X X X Standard with grid X X Cell+ X X X X Suspension- & hybridoma cultures X X X für Safe handling...

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Cell and Tissue Culture-9

Tissue Culture Plates Tissue Culture Plates with 3 growth surface options For optimized results Handy lateral grids in the base enable a safe grid of the whole plate Lids can only be attached in one direction 6 Well 96 Well 24 Well Wells with raised edges reduce the risk of contamination 6 Well Cell Culture Plates in new Multiwell Design with 3 different growth surfaces • Reduced risk of contamination during pipetting due to individual recessed wells • Clear viewing of media through transparent side panels • Minimized evaporation effects due to specially developed recessed lid with anti-condensation...

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Cell and Tissue Culture-10

Cell Scrapers • Coverslips Cell Scrapers with flexible 2-position blade More simple and efficient cell recovery by the thin and soft blade Depending on the application, the blade can be adjusted to “scrape” or “lift” cells from the growth surface. Sarstedt Cell Scrapers are used for the simple and gentle removal of the intact monolayers from tissue culture surfaces. • The blades of the new Sarstedt Cell Scrapers are produced from a more flexible, rubber-like, non-toxic material • The particularly thin, flexible blade and the handle from polypropylene are offered in different sizes, in order to...

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Cell and Tissue Culture-11

Roller Bottles For Research and Industrial Applications Expand your culture The ability of living cells to maintain their living functions and to produce biomolecules in a roller culture has 3 essential advantages compared to a static monolayer culture: • Enlarged growth surface, • Slow but steady mixing of the medium and • Enlarged medium surface in relation to the medium volume. Sarstedt Roller Bottles are produced from a special, virtually unbreakable material with excellent physical and chemical characteristics. Important construction features make the efficiency in performance of the Sarstedt...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.