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Freezing tubes

Freezing tubes

Freezing tubes

Product catalog summary
Introduction: This document provides guidelines for safely freezing samples using Sarstedt tubes, emphasizing the importance of proper storage techniques to prevent tube damage and sample loss.
Specifications: Plastic tubes, particularly those made from polycarbonate and polypropylene, offer high break resistance and impact strength compared to glass. However, their strength decreases at temperatures below 0°C, making them susceptible to cracking under mechanical stress.
Freezing Procedures: For safe freezing, it is crucial to freeze the content from bottom to top. Tubes should be stored upright in racks or boxes to allow cold air circulation and accommodate expansion. Screw caps are recommended for secure closure.
Avoidance Recommendations: Styrofoam containers are not ideal for deep-freezing due to their insulating properties, which can lead to uneven freezing and potential tube cracking. Overfilling tubes should be avoided to prevent increased internal pressure.
Freezing Dynamics: Aqueous solutions expand upon freezing, increasing pressure within the tube. Slow freezing methods, such as initial storage at -20°C before moving to -80°C, can help manage expansion and preserve tube integrity.
Conclusion: Due to varying stress factors, conducting a freezing test for specific applications is essential. Following the provided handling tips can minimize the risk of tube damage. Special CryoPure tubes are recommended for nitrogen storage, as they allow pressure adjustment during freezing and thawing.
Additional Resources: A summary of notes and the "Safety Instructions and User Guide CryoPure" can be downloaded from the Sarstedt website.
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Catalog excerpts

Freezing tubes-1

Tip of the month Freezing tubes Safe freezing – reliable storage

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Freezing tubes-2

Safe freezing – reliable storage The processing of your sample has worked perfectly and you have some more aliquots you would like to freeze at -80° C. Friday afternoon, 8 p.m., your experiment took more time than expected and your samples should be stored at -20° C over the weekend. The 1-litre bottle with media is rather inexpensive but also quite inconvenient to handle. What could be more logical than to store the media in smaller tubes in the fridge? How annoying if you notice after some days or weeks that your storage tube has leaked, cracked or broken completely. The sample is gone, your...

 Open the catalog to page 2
Freezing tubes-3

Safe freezing – reliable storage Tubes which are stored in liquid nitrogen can explode while thawing due to nitrogen expansion. Therefore, storing in the liquid nitrogen phase – also with a view to contamination risks – should be avoided. For the storage in the gaseous phase of nitrogen, the special CryoPure tubes can be used. The optimized cap design enables the pressure to adjust during freezing and thawing, and provides increased safety for nitrogen storage. Challenging compounds – what could be helpful? Some liquids tend to freeze very rapidly so that they expand very abruptly or irregularly....

 Open the catalog to page 3

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