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Growth Surface References

Growth Surface References

Product catalog summary

Introduction: The document provides comprehensive information on cell cultivation using Sarstedt's range of tissue culture (TC) flasks, dishes, and plates, which are designed with three distinct growth surfaces to cater to various cell types and ensure optimal growth conditions.

Growth Surface Options:

Sarstedt Standard Surface: This surface is designed for adherent cells, featuring hydrophilic groups to facilitate cell adhesion. It is color-coded red.
Sarstedt Cell+ Surface: Tailored for sophisticated adherent cells, such as primary cells and those cultured under serum-reduced conditions, this surface includes additional polar groups for enhanced adhesion and is color-coded yellow.
Sarstedt Suspension Culture Surface: Ideal for suspension cells, this hydrophobic surface minimizes cell losses due to unwanted microadhesion and is color-coded green.

Cell Cultivation Examples: Examples of various cell types cultivated on these surfaces are provided, demonstrating their vitality and suitability. For instance, HEK293 cells thrive on the standard surface, CHO cells on the Cell+ surface, and Jurkat cells on the suspension surface.

Cell Library: A comprehensive list of cell lines is included, detailing the suitable growth surface for each. This covers human, murine, and other species' cell lines, supporting a wide range of applications from cancer research to stem cell studies.

Recommendations: Users are encouraged to test the growth surfaces under their specific conditions to ensure optimal results. Sarstedt also invites users to share their experiences to expand the Cell Library.

Conclusion: Sarstedt's growth surfaces offer versatile solutions for cell cultivation, accommodating the diverse needs of different cell types. The color-coded system simplifies the selection process, ensuring researchers can easily identify the appropriate surface for their specific cell culture requirements.

Reference Library Overview: The document also serves as a reference library for various cell lines used in biomedical research, detailing their origins, types, and associated literature sources. Cell lines are categorized by species origin and cancer type, with references to studies utilizing these cell lines.

Key Sections:

Human Cell Lines: Lists numerous human cancer cell lines, including glioma, lung, colorectal, breast, prostate, and pancreatic cancer cell lines, with relevant literature references.
Murine Cell Lines: Includes mouse-derived cell lines such as fibroblasts, macrophages, and carcinoma cell lines, with study references.
Other Species Cell Lines: Covers cell lines from other species, including canine, rat, and monkey, used for various research purposes.
Primary Cells and Stem Cells: Lists primary cells and stem cells, highlighting their research use and associated studies.

Critical Information:

Cell Line Descriptions: Each cell line is described with its origin, type, and specific characteristics suitable for certain research types.
Literature References: Provides references to scientific articles where these cell lines have been used, offering a resource for researchers.
Applications: Cell lines are used in cancer research, drug testing, and cellular biology studies.

Conclusion: This reference library is a valuable resource for researchers in the biomedical field, providing detailed information on a wide array of cell lines and their applications in scientific research.

Additional Product Information: The document mentions bioreactors and cryopreservation systems, such as the miniPERM® bioreactor for protein production and the CryoPure Storage System for cryopreservation, highlighting their efficiency and reliability.

Contact Information: Sarstedt AG & Co. KG's contact details are provided for further inquiries, along with references to brochures related to cell and tissue culture, cryogenic storage, filtration, and liquid handling.

Catalog excerpts

Growth Surface References Which options do I have for the cultivation of my cells? Sterile/ DNA-/ DNase-/ RNase-/ Pyrogen-free/ non-cytotoxic Come Grow with us

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Are you planning to add a new cell line to your cell culture? Or cell growth could be optimized? No matter how different your cells are – their demands on the growth surface are just as individual. Our range of TC flasks, dishes and plates is therefore available with three different growth surfaces that come with a colour code for easy identification. For easy selection of the appropriate growth surface, the following pages provide an overview of cells that have already been successfully cultivated on our surfaces. This Cell Library is intended to support you in selecting the optimal growth surface...

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Growth surfaces & colour coding A basic requirement for the successful cultivation of cells in-vitro is to simulate the in-vivo environment of the relevant cell type as accurately as possible. The surface condition of the culture vessel is particularly important here, because many cell types can only survive, proliferate and differentiate following successful adhesion. In order to meet the requirements for as many different cell types as possible, Sarstedt offers flasks, dishes and plates with three different growth surfaces. The products are labelled as follows according to the Sarstedt colour...

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Cells cultivated on our three different growth surfaces Human bone osteosarcoma cell line Murine T-cell lymphoma cell line Murine mammary gland cell line Human kidney carcinoma cell line Human non-small cell lung cancer cell line Seabream vertebrae cell line (S. aurata) Human pancreas adenocarcinoma cell line Human pancreatic adenocarcinoma cell line Primary murine astrocytes B cell lymphoma cell line Murine melanoma cells Murine fibroblast cell line Bovine adrenal cortical capillary endothelial cells Human bronchial epithelium cell line Placental choriocarcinoma cell Placental choriocarcinoma...

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Cells cultivated on our three different growth surfaces Human foreskin fibroblasts Murine fibroblasts Murine pancreatic fibroblasts Murine mammary carcinoma cell line Human melanoma cell line Glioma Cells Primary human glioma cells Glioma Spheroids Human glioma spheroid culture Non-small cell lung cancer cell line (mucoepidermoid pulmonary carcinoma) Non-small cell lung cancer cell line (large-cell) Non-small cell lung cancer cell line (squamous cell carcinoma) Non-small cell lung cancer cell line (squamous cell carcinoma) Non-small cell lung cancer cell line (large-cell) Human keratinocyte cell...

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Cells cultivated on our three different growth surfaces Murine lung fibroblast cell line Murine fibroblast cell line (subcutaneous connective tissue) Murine fibroblast cell line (subcutaneous connective tissue) Lewis Lung Murine lung carcinoma cell line Rhesus monkey kidney epithelial cell line Human glioma cell line Human prostate carcinoma cell line Human colorectal adenocarcinoma cell line Luteinized granulosa cells Mesothelioma cell line Murine lymphoma cell line Fibrosarcoma cell line Human breast cancer cell line Human mammary adenocarcinoma Human breast cancer cell line Human breast cancer...

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Cells cultivated on our three different growth surfaces Rat non-cancer gastric epithelial cell line Rat pancreas cell line Rat lung epithelial cell line Myeloma cell line Human bladder carcinoma cell line Murine T-cell lymphoma cell line Schneider's Drosophila cell line (embryo epithelial) Human squamous cell carcinoma cell line Rat dental epithelial cells Fall armyworm ovary cell line Human neuroblastoma cell line Human breast cancer cell line Human adenocarcinoma cell line Non-small cell lung cancer cell line (squamous cell carcinoma) Human ovary adenocarcinoma cell line Murine mast cell cell...

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Reference Library... ...for cells cultivated on the standard adherent growth surface (red) Name Human bone osteosarcoma cell line Customer information/Sarstedt in-house test Murine mammary gland cell line Customer information/Sarstedt in-house test Human kidney carcinoma cell line Customer information/Sarstedt in-house test Human non-small cell lung cancer cell line Cytarska, J. et al., Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 70 No. 3 pp. 481ñ487, 2013 Human non-small cell lung cancer cell line Karimi-Busheri, F et al., Multidisciplinary Respiratory Medicine 2013, 8:65 Seabream vertebrae...

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Reference Library... ...for cells cultivated on the standard adherent growth surface (red) Name Human foreskin fibroblasts Customer information/Sarstedt in-house test Murine fibroblasts Customer information/Sarstedt in-house test Murine pancreatic fibroblasts Mueerkoester, S. et al., Cancer Research 64, 1331–1337, February 15, 2004 Human melanoma cell line Glioma Cells Primary human glioma cells Koschny, R. et al., Clin Cancer Res 2007;13:3403-3412 Non-small cell lung cancer cell line (Mucoepidermoid pulmonary carcinoma) Karimi-Busheri, F et al., Multidisciplinary Respiratory Medicine 2013, 8:65...

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Reference Library... ...for cells cultivated on the standard adherent growth surface (red) Name Murine lung fibroblast cell line Baig & Fish, Antiviral Therapy 2008 13:409-422 Murine fibroblast cell line (subcutaneous connective tissue) Baig & Fish, Antiviral Therapy 2008 13:409-422 Murine fibroblast cell line (subcutaneous connective tissue) Isakovic, A. et al., Toxicological Sciences 91(1), 173–183 (2006) Lewis Lung Murine lung carcinoma cell line Customer information/Sarstedt in-house test Rhesus monkey kidney epithelial cell line Customer information/Sarstedt in-house test Human glioma cell...

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Reference Library... ...for cells cultivated on the standard adherent growth surface (red) Name Murine embryonic fibroblast cell line Lührig, S. et al., Cell Division 2013, 8:3 Human gastric carcinoma cell line Customer information/Sarstedt in-house test Human osteosarcoma cell line Customer information/Sarstedt in-house test Human ovary adenocarcinoma cell line Customer information/Sarstedt in-house test Human ovary adenocarcinoma cell line Customer information/Sarstedt in-house test Human pancreatic carcinoma cell line Customer information/Sarstedt in-house test Human prostate adenocarcinoma...

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