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Catalogs
Sarstedt
PCR & Molecular biology

PCR & Molecular biology

Product catalog summary

Overview

This document provides detailed technical information on Sarstedt's liquid handling products, specifically designed for PCR and molecular biology applications. It includes product specifications, purity standards, compatibility tables, packaging options, and application recommendations.

1. Quality and Purity Standards

PCR Performance Tested Quality: Products are DNA-free, DNase/RNase-free, and PCR inhibitor-free with stringent limits on contaminant levels (Human DNA < 0.5 pg/μl, Bacterial DNA < 0.02 pg/μl, DNase < 1x10^-5 U/μl, RNase < 1x10^-9 Kunitz units/μl).
Biosphere® plus Quality: Enhanced purity including sterility, ATP-free, non-pyrogenic/endotoxin-free, with even lower contaminant thresholds (Human DNA < 5.0 fg/μl, Bacterial DNA < 0.2 fg/μl, DNase < 5x10^-7 U/μl, RNase < 5x10^-11 Kunitz units/μl, ATP < 1x10^-12 mmol/μl, Pyrogens < 0.002 EU/ml).
Sterility is validated according to ISO 11135 and conformity testing follows ISO 8655-2 standards.

2. Product Compatibility and Pipette Tips

A comprehensive compatibility table matches Sarstedt tips with various pipette types and volumes, indicating compatibility (●), limited volume use (10), or non-recommendation (✗).
Tip types include filter tips and tips without filters, available in multiple volumes ranging from 0.1 μl to 10 ml and beyond.
Packaging options include economical Tip StackPack, reload trays, and bulk-bagged tips suitable for autoclaving and manual racking.
Color-coded trays and order numbers facilitate easy identification and selection.

3. PCR Plates and Microplates

Various PCR plates are offered with different well numbers (96, 384), skirt types (full, half, none), and well profiles (high, low).
Plates are compatible with a wide range of thermocyclers from major manufacturers such as Applied Biosystems, BioRad, Eppendorf, Roche, and others, as detailed in the compatibility matrix.
Special features include fluorescence enhancement (10x higher fluorescence level), improved sensitivity, and reproducibility.
Packaging formats include bags, blister packs, and boxes with clear or white wells.

4. Tubes and Racks

Screw cap micro tubes designed for transport, storage, and sample preparation are compatible with a range of tube sizes in one rack.
Racks are rotatable, exchangeable, and suitable for temperature ranges from -80°C to 121°C.
Dimensions and ordering information for racks and tube handlers are provided.

5. Additional Products and Automation

ELISA plates for immunoanalytics and IVARO Tube Handler XS for automated tube processing are mentioned as part of the product portfolio.
Microbiology sample collection and processing products are also referenced.

6. Packaging and Ordering Information

Detailed order numbers, packaging units (units per bag, case), and color codes are provided for all product types.
Packaging options emphasize ease of use, space-saving, and environmental considerations.

Critical Information and Best Practices

Ensure selection of pipette tips compatible with your pipette model and volume range using the provided compatibility tables.
Use Biosphere® plus products for applications requiring the highest purity and sterility.
Follow sterility and purity standards to avoid contamination in sensitive PCR and molecular biology workflows.
Refer to compatibility charts for PCR plates to ensure thermocycler compatibility and optimal assay performance.
Utilize packaging options that facilitate sterile handling and reduce contamination risk.

Catalog excerpts

Liquid Handling

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Your Sarstedt Team Compatibility table

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PCR Performance Tested Quality • The Sarstedt quality and purity standard PCR Performance Tested ✓ DNA-free Human DNA < 0.5 pg/μl · Bacterial DNA < 0.02 pg/μl · DNase < 1x10-5 U/μl RNase < 1x10-9 Kunitz units/μl Conformity testing in accordance with the international test standard ISO 8655-2 Biosphere® plus quality • Unrivalled purity Biosphere® plus ✓ Sterile ✓ PCR inhibitor-free ✓ DNase/RNase-free ✓ Non-pyrogenic / endotoxin-free Human DNA < 5.0 fg/μl · Bacterial DNA < 0.2 fg/μl · DNase < 5x10-7 U/μl RNase < 5x10-11 Kunitz units/μl · ATP < 1x10-12 mmol/μl · Pyrogens < 0.002 EU/ml Sterilit

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Tailored tip solutions for every application

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Tip: to easily identify which tip is suitable for your pipette, use the compatibility table on pages 18-21or at www.sarstedt.

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Product (Illustrations: actual size) Tray colour code Purity Grade Tray colour code Type E

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Simple and practical Ec Economical and en environmentally friendly Tip StackPack packaging p Empty 96-tip reload trays and bulk-bagged tips Suitable for manually racking bulk tips for autoclaving and convenient handling. reducing packaging and storage space required in the Tip SystemBox Tray colour code Packaging Pcs./case Packaging Pcs./case Purity Grade

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Filter tips (Illustrations: actual size) Tray colour Order no. code Purity Grade Tips without filters (Illustrations: actual size) Tray colour code Type O Extra long illustration 1:2 ration at t Type C Type O Extra long illustration 1:2 tio ion io Purity Grade

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Quality Grade Units/bag Units/case Can be opened without touching the inside of the lid Quality Grade Units/bag Units/case Multiply® μStrip, suitable for Quality Grade

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Product description Packaging unit Ct values for the detection of plasmid copies in white and transparent plates at reduced reaction volumes White wells Transparent wells Number of plasmid copies • Fluorescence level 10x higher • Greater sensitivity • Improved reproducibilit

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Tip: to easily identify which plate is suitable for your thermocycler use our compatibility table on page 30/31 or at www.sarstedt.com. Units/bag Units/case Units/bag Units/case Blister packs/box Units/bag Units/case Units/bag Units/case Units/bag Units/cas

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Units/bag Units/case Units/bag Units/case Units/bag Units/case Units/bag Units/case Units/bag Units/case

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Number of wells Half/full skirt 96 pre-inserted Without High 384 full skirt Full - Number of wells Half/full skirt 96 pre-inserted Without High Amersham Biosciences® / GE Healthcare® BioRad®/MJ Research® Analytik Jena /Biometra 384 full skirt 96-well 384-well Full Full Full ‘Lightcycler’ ‘Lightcycler’ Corbett Research®/Qiagen® Applied Biosystems /Life Technologies ®

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Product description Special properties Clear Pierceable Functional temperature range Film/foil properties

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Experiment method: Volume Tubes with low binding properties Standard tube Purity Grade

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Units/bag Units/case Wavelength (nm) Units/bag Units/case Clear sides Units/Styrofoam rack/case

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Screw Cap Micro Tubes Sample collection, cultivation, processing Transport, storage and sample preparation Cell and tissue culture Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling Micro Test Plates for Immunoanalytics Designed to accommodate a range of tube sizes in one rack Units are rotatable and freely exchangeable Ideal for use in the temperature range from -80°C to 121°C L x W x H (per unit): 108 x 108 x 257 (108 x 108 x 69) mm Revolver Workstation Innovative automation for tube processing ELISA Plates Revolver Workstation IVARO Tube

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All Sarstedt catalogs and technical brochures

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