Appl_Biostat-A_SBT1024 - 4 Pages

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Appl_Biostat-A_SBT1024

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sartoriusstedim biotech Propionic acid production of Propionibacterium acidipropionici in a BIOSTAT® A turning science

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Carlos H Luna-Flores, Lars Nielsen and Esteban Marcellin* Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Qld 4072, Australia * Corresponding author Dr. Esteban Marcellin, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia. Phone: +61 7 334 64298, Fax: +61 7 3346 3973, Email: e.marcellin@uq.edu.au Introduction Traditionally derived from fossil fuels, propionic acid (PA) is widely used in the food and pharmaceutical industries. PA is used as a preservative and...

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1.3. Analytical methods Optical density of the culture was measured at 600 nm using a Biochrom Libra S12 UV/Vis Spectrophotometer. Organic acids and carbohydrates were quantified by ion-exclusion chromatography using an Agilent 1200 HPLC system and an Agilent Hiplex H column (300 × 7.7 mm, PL1170-6830) with guard column (SecurityGuard Carbo-H, Phenomenex PN: AJO-4490). Sugars were monitored using a refractive index detector (Agilent RID, G1362A) set on positive polarity and optical unit temperature of 40°C, while organic acids were monitored at 210 nm (Agilent MWD, G1365B). 30 µL of sample...

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For further contacts, visit www.sartorius-stedim.com Europe Americas Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen Phone +49.551.308.0 Fax +49.551.308.3289 Sartorius Stedim Systems GmbH Robert-Bosch-Strasse 5 - 7 34302 Guxhagen Phone +49.5665.407.0 Fax +49.5665.407.2200 Netherlands Sartorius Stedim Netherlands B.V. Phone +31.30.60.25.080 Fax +31.30.60.25.099 filtratie.nederland@sartorius-stedim.com Poland Sartorius Stedim Poland Sp. z o.o. ul. Wrzesinska 70 62-025 Kostrzyn Phone +48.61.647.38.40 Fax +48.61.879.25.04 USA Sartorius Stedim North America...

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