BioPAT® Trace - 4 Pages

Add to MyMedicalExpo favorites
BioPAT®  Trace

Catalog excerpts

sartoriusstedim biotech BioPAT® Trace Glucose | Lactate Monitoring turning science

 Open the catalog to page 1

Ajith T. George+, Anil Kumar Rathod1', Dr. Ashok Mundrigi1', Dr. Andreas Prediger2', Dr. Stuart Tindal2' 11 Sartorius Stedim India Pvt. Ltd., Bangalore, India 2) Sartorius Stedim Biotech GmbH, Goettingen, Germany 1. Introduction To determine the state of biotechnological cell cultivations it is important to monitor the concentration of key substrates and metabolites such as glucose and lactate. The BioPAT® Trace, a device designed for online monitoring of these parameters, was evaluated during 3 Fed-Batch cultivations of an industrial CHO (Chinese Hamster Ovary) cell line used for IgG...

 Open the catalog to page 2

The profiles of glucose and lactate concentration during the cultivation are shown in figure 3. Online Glucose & Lactate Estimation Batch No. 1 18 Figure 1: BioPAT® Trace and buffers connected with a dialysis probe to a bioreactor. Viable cell density (x106 cells/mL) Growth Profile of CHO cell line in Fed batch mode Batch Age, Days Batch 2 Cell density Batch 3 Cell density Figure 2: Overview of the growth patterns of the performed CHO cell cultivations. Batch 1 Cell density During the cultivations, peak cell densities of almost 30 × 106 cells/ml were achieved after 9 – 10 days. During that...

 Open the catalog to page 3

The lactate concentration increased proportionally to the cell density & reaches a maximum of 2.0 – 3.0 g/L after 4 days. Afterwards, it gradually decreases to a level of less than 0.5 g/L. A calibration and referencing procedure of the BioPAT® Trace was performed at the start of the cultivations and during cultivation when the estimation of the device deviated from the reference measurement (indicated with black arrows). Measured glucose concentration [g/l] In figure 4 the measured concentration values from the BioPAT® Trace are compared to the reference values. 4. Conclusion The BioPAT®...

 Open the catalog to page 4

All Sartorius Group catalogs and technical brochures

  1. Practum®

    8 Pages

  2. Quintix®

    12 Pages

  3. Biostat®A

    2 Pages

  4. DoE

    4 Pages

  5. EVOLVING

    8 Pages

  6. BIOSTAT® A

    7 Pages

  7. BIOSTAT® RM

    6 Pages

  8. BIOSTAT STR

    24 Pages

  9. fill-it

    4 Pages

  10. ambr® 15

    4 Pages

  11. ambr® 250

    4 Pages

  12. Sartobind®

    10 Pages

  13. FlexAct®

    16 Pages

  14. BioPAT®

    8 Pages

  15. Bioprocess

    768 Pages

  16. BACTair™

    8 Pages

  17. Vivaspin 500

    94 Pages

  18. Sartobran

    2 Pages

Archived catalogs