BIOSTAT® Aplus bioreactor - 3 Pages

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BIOSTAT® Aplus bioreactor

Catalog excerpts

VWRbioMarke" THE MARKET SOURCE FOR LIFE SCIENCE Batch and fed batch cultivation of different mammalian cell lines in the BIOSTAT® Aplus bioreactor D . Lampe, B . Putz, D . Lutkemeyer , Frank Gudermann, Apparative Biotechnology, Department of Engineering Sciences and Mathematics, Bielefeld university of applied Sciences, Bielefeld, Germany Sabrina Armgart, ute Noack, Sartorius Stedim Biotech GmbH, Goettingen, Germany BIOSTAT® Aplus is a compact laboratory bioreactor with an autoclavable vessel featuring a stirred tank design. the system is available in a choice of culture 1, 2 and 5 litre volumes and preconfigured versions for microbial and cell cultivation. This wide selection makes it easy for users to choose the bioreactor model that best matches their specific needs . Thanks to the intuitive operating design of this bioreactor via notebook PC, it is especially suitable for educational purposes or first time bioreactor users . Typical applications range from small scale protein expression to up-scale experiments from uncontrolled shake or T-flasks to controlled cultivation conditions . All systems measure and automatically control dissolved oxygen (DO), pH, temperature, foam and level . in particular, DO control can be set-up as in larger scale bioreactors normally used in process development, while keeping operation easy. Stirrer speed, gas mixing and substrate addition can be selected for the DO cascade . Automatic pH control can be accomplished by selecting acid and base addition or CO2 and base addition to suit the needs of a specific application . Three internal peristaltic pumps can be used for corrective agents or substrate . A fourth external pump can also be connected . Different impeller and sparger designs are available to suit organisms with a high demand for oxygen as well as sheer sensitive cells . The BiOSTAT® ApIus also includes an installation video and cultivation recipes for common cell lines to facilitate installation and first time use of the bioreactor. The following application data was compiled at the University of Applied Sciences in Bielefeld, Germany. At Bielefeld University the BiOSTAT® Aplus is used for various applications during students' practical lab course work in cell cultivation . 1st application: CHO cultivation in fed batch mode For fed batch cultivations in a 2 litre BiOSTAT® Aplus system, a CHO cell line was used which secretes a recombinant monoclonal igG antibody. The protein-free, chemically defined medium, MAM-PF 2 from BioConcept Amimed, was used as the culture medium . The concentration of living and dead cells was measured using the Cedex from Roche Applied Sciences . The concentration of glucose, lactate, glutamine, ammonium and igG was determined using the Cubian XC from Optocell . (Figure 1) The bioreactor was equipped with a 3 blade segment impeller with pull down mixing direction in the upper section and a 6 blade disc (Rushton) impeller approx . 2 cm above the sparger. At an agitation speed of 200 rpm, this configuration — in combination with the microsparger used for the submerse gassing — ensures a small gas bubble diameter as well as good mixing conditions . The residence time of the small gas bubbles in the medium is also 30 I VWR INTERNATIONAL I VWRbioMarke Issue 27 I September 2011

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CELL BIOLOGY For more information on these products contact your local VWR sales office, send an e-mail to or visit our website increased, as they are retained for some time in the lower section of the bioreactor due to the flow profile of the disc impeller. The regulation of the pH at 7,1 using CO2 was performed exclusively via the headspace . A constant flow of air (200 ccm) through the headspace was mixed with the corresponding proportion of CO2 via the control of the Aplus . Using sodium carbonate (1 M), the pH could, if necessary, be maintained in the...

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the market source for life science The viable cell concentration reached 56 ∙ 105 cells/ml with a viability of 98%. The specific growth rate was 1.06 d-1. At the end of the process, the product concentration was 35 mg/l (referred to as 100% in Figure 3). (Figure 4) In order to continuously determine the concentration of viable cells, the capacity of the cell suspension was measured using the i-Biomass 465. Figure 4 shows that, in the first 5 days of the cultivation, the capacity correlates very well with the concentration of living cells determined offline. After the third addition of the...

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