BIOSTAT ® CultiBag STR 50 plus MO - 8 Pages

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BIOSTAT ® CultiBag STR 50 plus MO

Catalog excerpts

sartoriusstedim biotech High cell density Escherichia co/z cultivation in the stirred single use bioreactor BIOSTAT® CultiBag STR 50 plus MO turning science

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Thomas Dreher, Ute Husemann, Gerhard Greller, Jorg Weyand Sartorius Stedim Biotech, GmbH, August-Spindler-Str.11, 37079 Goettingen, Germany Single use products offer many advantages in manufacturing processes, due to this they have become widely accepted for cell cultural applications (Eibl et al., 2009). Nowadays, reports about microbial application are rarely found in single use bioreactors, commonly because the oxygen transfer is a limiting factor. The BIOSTAT® CultiBag STR 50 plus MO is a single use bioreactor, which is specially designed to fulfil the requirements of...

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The BIOSTAT ® CultiBag STR 50 plus MO (see figure 1) is a further development of the well established STR 50 cell culture version. The stirrer speed and therefore the power input have been increased compared to the basis system. Furthermore, the aeration rates are adapted to microbial requirements. As a consequence, the oxygen transfer of this bioreactor is significantly improved. Volumetric mass transfer coefficients (kLa) above 150 h-1 can be achieved with this bioreactor (data Sartorius Stedim Biotech). 2. Material and methods 2.1 Bacterial strain For the cultivation Escherichia coli...

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For the fermentation of the main culture a BIOSTAT ® CultiBag STR 50 plus MO with a double wall system was used. The temperature was controlled by the cooling unit Frigomix 2000 (Sartorius Stedim Biotech). The used CultiBag STR 50 had the configuration 6-blade-disk impeller (bottom) and a 3-blade-segment impeller (top). For the aeration a ring sparger with 5 holes and a diameter of 0.8 mm was installed. Depending on the oxygen demand the agitation rate was varied between 250 – 370 rpm and the aeration rate between 5 – 20 Lpm. If necessary, pure oxygen was supplied. The kLa-value for these...

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A constant µ of 0.4 h-1 was determined for both systems during the batch phase (0 – 12 h for the C+, 0-9 h for the STR plus MO). This corresponds to the maximal specific growth rate at 28°C (Dreher, 2010). After the feed start µ decreased. It was possible to control the specific growth rate to 0.1 h-1. Feed start Figure 3: rowth behaviour during the Escherichia coli cultivations in G the BIOSTAT ® CultiBag STR 50 plus MO and BIOSTAT ® C+. The characteristics of the OD600 and specific growth rate are shown. To guarantee ideal growth behaviour it is necessary to control the cultivation...

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For the measurement of the pH and pO2 two different measuring principles were used. In figure 5 the measured data of the optic chemical and classical sensors are represented. The values for the pO2 as well as the pH of both measuring principles showed only low deviations. glucose acetate pO 2 optical pH classical pH optical To make conclusions about the metabolic activity during the cultivations the glucose and acetate concentrations were determined (see figure 6 a,b). The initial glucose concentration was 9 g/L for the STR 50 plus MO and 9.8 g/L for the C+. At the end of the batch phase...

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4. Discussion The BIOSTAT ® CultiBag STR 50 plus MO is a modified version of the well established cell culture bioreactor. It is a single use bioreactor, which is engineered completely on principles known from reusable bioreactors. To examine this system for microbial suitability an Escherichia coli fed batch cultivation was carried out. A cell density of OD600 = 160 (DCW = 60.8 g/L) was achieved. This is comparable to industrial E. coli cultivations in stainless fermenters. Hence, the results show that microbial high cell density cultivations are possible in the BIOSTAT ® CultiBag STR 50...

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Sales and Service Contacts For further contacts, visit www.sartorius-stedim.com Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen Denmark Sartorius Stedim Nordic A/S Hoerskaetten 6D, 1. DK-2630 Taastrup USA Sartorius Stedim North America Inc. 5 Orville Drive Bohemia, NY 11716 Australia Sartorius Stedim Australia Pty. Ltd. Unit 5, 7-11 Rodeo Drive Dandenong South Vic 3175 Hungary Sartorius Stedim Hungária Kft Kagyló u. 5 2092 Budakeszi Sartorius Stedim SUS Inc. 1910 Mark Court Concord, CA 94520 China Sartorius Stedim Beijing Representative Office No. 33, Yu’an...

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