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DoE - 4 Pages

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DoE

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sartoriusstedim biotech Design of Experiments for optimal production of malaria vaccines with Pichia pastoris turning science

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H AW – Hamburg University of Applied Sciences, Germany 2 S artorius Stedim Biotech GmbH, Germany 3 B PRC – Biomedical Primate Research Centre, The Netherlands Application background The aim of a joint project of the HAW Hamburg and the Biomedical Primate Research Center (BPRC) was to identify optimal protein expression conditions for high yields of potential malaria vaccines in the methylotrophic yeast Pichia pastoris. Optimal expression conditions vary in accordance to the kind of Pichia pastoris strain used for the foreign protein expressed. As strain characterization and target protein...

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Investigated expression factors and quality responses As a first step a DoE screening procedure focused on the impact of three parameters, the methanol concentration in the media phase cS2M, the pH-value, and the liquid temperature ϑL during expression. By varying these process parameters with DoE it is expected to improve target protein yield with minimum proteolytic degradation. Results of the DoE-screening Figure 4 shows the results of the DoE-screening which was accomplished by using a full factorial design. The lower response surface represents the productivity at pH 4, the upper at pH...

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For further contacts, visit www.sartorius-stedim.com Europe Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen Phone +49.551.308.0 Fax +49.551.308.3289 www.sartorius-stedim.com Hungary Sartorius Stedim Hungaria Kft Kagylo u. 5 2092 Budakeszi Phone +36.23.457.227 Fax +36.23.457.147 America USA Sartorius Stedim North America Inc. 5 Orville Drive, Suite 200 Bohemia, NY 11716 Toll-Free +1.800.368.7178 Fax +1.631.254.4253 India Sartorius Stedim India Pvt. Ltd. #69/2-69/3, Jakkasandra Kunigal Road, Nelamangala Tq Bangalore - 562 123 Phone +91.80.4350.5248 Fax...

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