NucleoCounter® NC-250? Viability and Cell Count Assay for easy, fast and objective cell counting - 4 Pages

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NucleoCounter® NC-250? Viability and Cell Count Assay for easy, fast and objective cell counting

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NucleoCounter® NC-250™ Viability and Cell Count Assay for easy, fast and objective cell counting Product Information The NucleoCounter® NC-250™ used together with high quality glass slides enables fast and highly precise determination of cell count and viability at an extremely low price per count. It is highly suitable for all mammalian cell types whether it is a suspension, adherent or primary cell line. The NucleoCounter® NC-250™ uses 2 and 8 chamber slides made of high quality glass. Add solution 18 to your cell sample, load the slide and run it. Within 3 minutes you will have 8 viability and cell counts including the number of viable, non-viable and total cell concentration as well as the viability percentage and an estimated cell diameter. Solution 18 contains the fluorescent cell dyes acridine orange and DAPI for total and dead count determination respectively. Principle Using fluorescence microscopy and image analysis, the NucleoCounter® NC-250™ automates the detection of cells. The NC-250™ Viability and Cell Count Assays use the cell stain Acridine Orange for cell detection, and the nucleic acid stain DAPI for detecting non-viable cells. DAPI cannot penetrate the cell membrane, hence it only stains cells with a permeable cell membrane; the non-viable cells. The different Viability and Cell Count Assays are optimized for viability and cell counting of mammalian and insect cells, e.g. leucocytes in whole blood samples. For high precision use the NC-Slide A2™ and for screening use the NC-Slide A8™. Key Benefits – erform 8 viability counts in less than 3 minutes! P – xtremely fast and convenient measurement of cell count E and viability – igh accuracy and high reproducibility H – utomatic data collection A – bjective analysis O – o calibration required N – pecialized protocols for Purified Leucocytes and S Full Blood Samples. – lot Manager for superior data presentation P – xport of data in FCS/ACS formats E Cell Counting

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Analysis High Precision Analysis High Speed Analysis Harvest Cells Harvest Cells Load Cells into NC-Slide A2™ Load Cells into NC-Slide A8™ Image Cytometry Image Cytometry Principle Using fluorescence microscopy and image analysis, the NucleoCounter® NC-250™ automates the detection of cells. The NC-250™ Viability and Cell Count Assays use the cell stain Acridine Orange for cell detection, and the nucleic acid stain DAPI for detecting non-viable cells. DAPI cannot penetrate the cell membrane, hence it only stains cells with a permeable cell membrane; the non-viable cells. NC-Slide A8™ (8...

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A: Viability and Cell Count Image B: Results from NC-250™ Report Generator ansa Hri^^Miui^tiHauuhil Result of Viability and Cell Count Assay (A) Image showing a selection of the cells measured. All cells are stained with Acridine Orange (AO) represented by green cells. Nonviable cells are stained with DAPI represented by blue cells. (B) Report Generator showing results from the Viability and Cell Count. Cell Counting

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Sales and Service Contacts For further contacts, visit www.sartorius-stedim.com Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen Italy Sartorius Stedim Italy S.p.A. Via dell’Antella, 76/A 50012 Antella-Bagno a Ripoli (FI) USA Sartorius Stedim North America Inc. 5 Orville Drive, Suite 200 Bohemia, NY 11716 Sartorius Stedim Systems GmbH Robert-Bosch-Strasse 5 – 7 34302 Guxhagen Netherlands Sartorius Stedim Netherlands B.V. Argentina Sartorius Argentina S.A. Int. A. Ávalos 4251 B1605ECS Munro Buenos Aires Phone +49.5665.407.0 Fax +49.5665.407.2200 France...

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