Online Glucose | Lactate measurement - 4 Pages

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Online Glucose | Lactate measurement

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sartoriusstedim biotech Online Glucose | Lactate measurement in Batch and Fed-batch CHO cultivation turning science

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Cell number | Viability 120.00 Cell number [cells/ml] E+05 Figure 1: Cell numbers and viability during the batch culture Comparison CuBiAn and BioPAT® Trace 3 A CHO cell line was used for the cultivation in a chemical defined, protein free media (Mam-pf2, Bioconcept). The cultivations were performed in a Biostat® A which was online connected to the BioPAT ® Trace and controlled by the BioPAT ® MFCS (all systems from Sartorius Stedim Biotech GmbH). For the control measurements of glucose and lactate a CuBiAn XC (Optocell) was used. The cell numbers and viabilities were measured with a Cedex...

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The demonstrated results show the ability of online measurement of glucose and lactate during the cultivation of CHO cells with the BioPAT ® Trace system. Other the whole cultivation time a stable measurement could be observed. Cell number and viability Fed-Batch 250.00 Cell number [cells/ml] E+05 The easy handling of the system gives the user the possibility for an easy integration of this online measurement in every existing cultivation system. The online measurement offers the possibility to measure the glucose and lactate values with a time dependent strategy. During the presented...

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Sales and Service Contacts For further contacts, visit www.sartorius-stedim.com Asia| Pacific Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse 11 37079 Goettingen Denmark Sartorius Stedim Nordic A/S Hoerskaetten 6D, 1. DK-2630 Taastrup USA Sartorius Stedim North America Inc. 5 Orville Drive Bohemia, NY 11716 Australia Sartorius Stedim Australia Pty. Ltd. Unit 5, 7-11 Rodeo Drive Dandenong South Vic 3175 Hungary Sartorius Stedim Hungária Kft Kagyló u. 5 2092 Budakeszi Sartorius Stedim SUS Inc. 1910 Mark Court Concord, CA 94520 China Sartorius Stedim Beijing Representative Office...

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