Ultrafiltration Family
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Catalog excerpts

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Simplifying Progress

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General Information Major Uses for Ultrafiltration 4 Sartorius Lab Ultrafiltration Devices 5 Membrane Performance Characteristics 6 Membrane Selection Guide 7 Protein and Macromolecule Concentration 9 Centrifugal Filtration ■ Vivaspin® 500 10 Tangential Flow Filtration Vivaflow® 50 36 Solvent Adsorption ■ Vivapore® 5, 10 | 20 44 Ultrafiltration Membrane Filters from PES 146..., CTA 145... and RC 144... 46

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Vivapure® Ion Exchange Protein Purification Products 58 Virus Purification and Concentration 61 Vivapure® Virus Purification and Concentration Kits 62 Adenovirus Purification with Vivapure® AdenoPACK Kits 63 Lentivirus Purification with Vivapure® LentiSELECT Kit 68 1. Desalting and Buffer Exchange with Vivaspin® Centrifugal Concentrators 74 2. Treatment of Vivaspin® Concentrators for Improved Recovery of Low-concentrated Protein Samples 77 3. Scouting Protein Purification Conditions Using Vivapure® Centrifugal Ion Exchange Membrane Absorbers 80 4. Sartorius Ultrafiltration Products for the...

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Ultrafiltration is a convective process that uses anisotropic semi-permeable membranes to separate macromolecular species and solvents primarily on the basis of size. It is particularly appropriate for the concentration of macromolecules and can also be used to purify molecular species or for solvent exchange. Ultrafiltration is a gentle, non denaturing method that is more efficient and flexible than alternative processes Typical applications for ultrafiltration ■ Concentration | desalting of proteins, enzymes, DNA, monoclonal antibodies, immunoglobulins, extracellular vesicles , viruses,...

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Sartorius develops devices dedicated for optimising laboratory ultrafiltration processes for the purpose of minimising processing times and maximising recovery, reliability and robustness. In addition Sartorius are continually building technical and application Sartorius offers a comprehensive range of process alternatives for the filtration and concentration of biological samples. Below is a guide to selecting the most suitable filtration method, depending on sample volume, equipment available, filtration speed and process control desired. Centrifugal concentrators (100 pL to 100 mL...

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Sartorius offers an extended range of membranes to cover the great majority of ultrafiltration requirements. The following is a guide to selecting the most appropriate membranes according to their typical performance characteristics. Please note however, that membrane behavior and ultimate performance, largely depends on the specific characteristics of the solution being processed. Sartorius recommends that users experiment with alternative membranes in order to optimise their process performance. Polyethersulfone (PES) This is a low binding membrane that provides excellent performance with...

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The advanced designs and low adsorption materials that characterise Sartorius products, offer a unique combination of faster processing speeds and higher recovery of the concentrated sample. Providing that the appropriate device size and membrane cut-off is selected, Sartorius products will typically yield recoveries of the concentrated sample in excess of 90% when the starting sample contains over 0.1 mg/mL of the solute of interest. The majority of any loss is caused by non specific binding both to the membrane surface and to exposed binding sites on the plastic of the sample container:...

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Chapter  Site Title

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Ultrafiltration Membrane Filters from PES 146..., CTA 145... and RC 144... 46

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Vivaspin® 500 100 μL to 500 μL samples Vivaspin® 500 μL centrifugal filter units offer a simple, one step procedure for sample preparation. They can effectively be used in a fixed angle rotors accepting 2.2 mL centrifuge tubes. The legacy patented vertical membrane design and thin channel filtration chamber (US 5,647,990), minimises membrane fouling and provides high speed concentrations, even with particle laden solutions. Technical specifications Vivaspin® 500 Concentrator capacity Swing bucket rotor Fixed angle rotor Dimensions Total length Active membrane area Hold-up volume, membrane...

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Performance characteristics Time to concentrate up to 30x [min.] at 20°C and solute recovery % Rotor Fixed angle Ordering information Vivaspin® 500 Polyethersulfone Pack size Starter pack (5 of each 5 k, 10 k, 30 k, 50 k, 100 k) 25 Protein Concentration Centrifugal Filtration Vivaspin® 500

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Vivaspin® 2 Choice of membranes 0.4-2 mL samples The Vivaspin® 2 bridges the gap between the 500 gL and 4 mL centrifugal concentrators. This device combines the speed of the classic Vivaspin® products with low internal surface and membrane area for superior recoveries from very dilute solutions. Available with a choice of PES, Cellulose Triacetate and Hydrosart® membranes, Vivaspin® 2 offers the highest flexibility for process optimisation. Technical specifications Vivaspin® 2 Also unique to the Vivaspin® 2, is the choice of directly pipetting the concentrate from the dead stop pocket built...

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Integral deadstop avoids risk of concentrating to dryness Time to concentrate up to 30x [min.] at 20°C and solute recovery % Rotor Fixed angle PES, CTA, or Hydrosart® membranes; Filtrate container fits standard 15 mL tube carriers Direct pipette recovery or choice of reverse spinning concentrate into sample cap Protein Concentration Centrifugal Filtration Vivaspin® 2

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Ordering tips ■ Choose a membrane pore size at least 50% smaller than the size of the molecule to be retained. ■ Usually choose Polyether-sulfone membranes for fastest concentrations. ■ Usually choose Cellulose Triacetate for Protein Removal | Ultrafiltrate recovery. ■ Usually choose Hydrosart® membranes for highest recovery with Ig fractions. Ordering information Vivaspin® 2 Polyethersulfone 3,000 MWCO 3,000 MWCO 5,000 MWCO 5,000 MWCO 10,000 MWCO 10,000 MWCO 30,000 MWCO 30,000 MWCO 50,000 MWCO 50,000 MWCO 100,000 MWCO 100,000 MWCO 300,000 MWCO 300,000 MWCO 1,000,000 MWCO 1,000,000 MWCO 0.2...

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