UniSart ® Nitrocellulose Membranes
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UniSart® Nitrocellulose Membranes The Substrate of Choice for Protein Assays 1

Sartorius is a leading international provider of integrated solutions for the biopharmaceutical industry. With our extensive portfolio of products, technologies and services, we cover wide areas of the biopharmaceutical process chain, ranging from fermentation, cell cultivation, filtration and purification to media storage and transportation. Being the first industrial manufacturer of nitrocellulose membranes, Sartorius has developed an unmatched expertise in the production of various high quality nitrocellulose matrices. … Committed to the Industry Sartorius is the partner for developers...

Nitrocellulose The Substrate of Choice for Protein Assays The popularity of the UniSart® nitrocellulose membranes as a major component of many rapid tests has been built on their prominent features. Features & Benefits Availability on Large Scale Wide Range of Applications High Protein Binding 3D Structure The 3D microporous matrix allows liquids to flow in all directions High Consistency High inter- and intra- lot consistency Excellent Performance Complete Traceability Reliable Supply Quality made in Germany

The UniSart® Product Portfolio UniSart® CN Membranes for Lateral Flow Tests When reliable capillary speed, homogenous thickness and defect‑free surface qualility need to be combined to obtain high quality lateral flow tests, then the UniSart® CN 95, CN 110, CN 140, CN 150 and CN 180 DX are the membranes of choice. Please find more details and ordering information on page 8. 6 UniSart StructSure® Membranes for Multiplex Assays These structured membranes have been developed through an innovative patterning process in order to better fulfill the need for rapid multiparamater tests. Please find...

UniSart® CN Membranes for Lateral Flow Tests Membranes for Lateral Flow Tests The UniSart® membranes with large pores have been designed to be the best substrate for the 3 billion and more rapid lateral tests manufactured and used every year around the world. complex can be captured by the reagents that are nested on the test and control lines. The UniSart® membranes have been developed to offer the features essential for highly reproducible readouts. with high consistency. From many square meters of one batch down to the few square centimeters of a lateral strip, our stringent QC and...

Specifications & Characteristics Choose the Best Membrane for your Application cellulose nitrate cellulose nitrate cellulose nitrate cellulose nitrate cellulose nitrate 100 μm polyester, clear; 100 μm polyester, white Precise amount of anionic surfactant allows for fast wetting Background clearing very fast very fast most suitable suitable, depending on migration distance suitable, depending on migration distance suitable, depending on migration distance suitable, depending on migration distance suitable, depending on migration distance very good very good very good very good, very high...

Ordering Information UniSart® Membrane Polyester Backing Catalog Number UniSart® Membrane Polyester Backing Catalog Number Customized dimensions of UniSart® CN membranes are also available upon request. For further information and customized dimensio

UniSart StructSure® Membrane The innovative way to build your own multiparameter assay on nitrocellulose membrane with microfluidic design Up to now, the features of nitrocellulose membranes have been essentially exploited in simple longitudinal strip or vertical flow through devices where the membrane is limited to simple rectangular or circular shape. Sartorius has now developed a new technology to engraving different patterns into a nitrocellulose membrane. This innovative process leads to the creation of a hydrophobic barrier delineating the membrane zones. The remaining membrane stays...

Significant decreased signal intensity on capture spots of row A compared to row B on UniSart® CN 140 membrane Capture spots show similar signal intensity on both rows with a UniSart StructSure® membrane Example 2 In this second example, the test spots are in the first row and the control spots are in the second row of the strips. The capture spots positioned on the UniSart StructSure® membrane are showing increased intensity compared to the ones on the UniSart® membrane. Indeed, the restrictions in the liquid flow brought by the addition of individual lanes are assisting the binding of the...

UniSart® Membranes for Immunoassay and Blotting Immunoassay Line or dot blot strips are widely used for in vitro diagnostic tests. Many infectious diseases generate a complex immune response and require an antibody profiling of the patient serum. To do so, the different immuno-dominant antigens have to be applied in line or dots onto a uniform binding substrate. Today, long after the first membrane was used in blotting, the nitrocellulose membrane is still the best substrate for proteins. The UniSart® 0.22 μm and 0.45 μm nitrocellulose membranes have been designed to show exceptional...

Typical Parameter Value of the UniSart® 0.22 μm and 0.45 μm UniSart® Blotting Membrane Flow rate for water [ml/(min. cm2 bar)] Bubble point with water [bar] Extractable content in water [%] Wetting with water [secs] Membrane wound with yellow interleaving protection paper between the membrane layers. UniSart StructSure® membranes can also be designed on small pore size nitrocellulose to create multiplex blot cards to better distinguish each test zone. Ordering Information UniSart® CN Blotting Membrane Backing Polyester Catalog Number Customized dimensions of UniSart® CN membranes are also...

UniSart® Microarray Slides UniSart® Microarray slides are engineered for consistent protein microarrays UniSart® Microarray slides For the new generation of protein arrays, Sartorius has developed glass slides coated with a thin microporous nitrocellulose membrane. Nitrocellulose membranes are the substrate of choice when it comes to protein-based assays. Widely used in electrophoresis blotting techniques and new rapid diagnostic immunoassays, the classical nitrocellulose membranes needed to be reengineered to best fit the protein array applications. The UniSart® Microarray slides have an...