Catalog excerpts
Product Datasheet Unique, plug and play laboratory crossflow cassettes Product Overview Rapid sample processing, with ready-to-use cassettes operated by a standard peristaltic pump Plug and play simplicity, thanks to the unique flip-flow channels that ensure optimal flux Flexible and modular scalability, with a broad choice of membrane, MWCO and single or multi use options for samples up to 5 L Vivaflow® brings the benefits of tangential flow filtration to research and development laboratories. Operated with a standard peristaltic pump, these ready-to-use cassettes eliminate the cost and complexity of process-scale systems. Ideal for the ultrafiltration and diafiltration of 0.1 to 5 L samples, the unique flip-flow channel design provides plug and play convenience, ensuring optimal crossflow velocities for fast concentr
Open the catalog to page 1Product Information The Vivaflow® range offers a choice of crossflow devices for scientists who need to reliably concentrate or re-buffer aqueous samples with initial volumes of up to 5 L. Unlike other crossflow cassettes on the market, Vivaflow® is a dedicated laboratory product that meets the demand for ease of use without requiring additional, non-standard equipment or significant process optimization. A choice of devices, operated with a standard peristaltic pump, achieve optimal results for every ultrafiltration need: Vivaflow® cassettes lend themselves to a multitude of...
Open the catalog to page 2Technical Data Vivaflow® 50 Main housing Flow channel Membrane support Membrane seals and O rings Pressure indicator Flow restrictor Overall L | H | W Channel W | H Active membrane area Hold-up volume, cassette Non-recoverable hold-up Pump flow rate Maximum pressure Maximum temperature Operating Conditions * Pressure indicator is available separately (Order no. VFA020) as an optional accessory for Vivaflow® 50 ** SS = stainless steel **
Open the catalog to page 3Working Principle Convenient Diafiltration Vivaflow® cassettes contain an ultrafiltration membrane over which a sample is recirculated using a peristaltic pump (Figure 1). The thin channel flip-flow path provides high crossflow velocities with minimal pump speed requirements of 200 – 400 mL/min. A flow restrictor included with each cassette generates the optimal transmembrane pressure for concentration | diafiltration of the sample, whilst the filtrate is collected in a separate vessel. Ultrafiltration can be stopped as soon as the desired volume is reached by simply turning off the pump....
Open the catalog to page 4Components for Operating One to Six Vivaflow® 50 Cassettes Figure 4 Precise scale-up in process speed when using one to four Vivaflow® 50 cassettes. 4x cassettes The modular design of Vivaflow® 50 enables the operation of multiple cassettes in series and parallel (Figure 3 and Table 1). This increases the maximum throughput to up to 3 L and accelerates processing speed in proportion to the membrane area (Figure 4). Concentration Factor Time [hr:min] Figure 3 System components for operating multiple Vivaflow® 50 cassettes. Series interconnectors (Order no. VFA031) T-connectors (Order no....
Open the catalog to page 5Components for Operating One or Two Vivaflow® 50R | 200 Cassettes The maximum throughput of Vivaflow® 50R | 200 can Sample Feed also be increased and processing time reduced| by adding a second cassette to the setup (Figure 5 and Table 2). Figure 5 System components for operating two Vivaflow® 50R | 200 cassettes For Vivaflow® 50R: T-connectors to | from inlets | outlets (Order no. VFA030) For Vivaflow® 200: Y-connector to inlets (Order no. VFA005) Masterflex pump and Easy-Load pump head (Order no. VFP001 | VFP002 and VFA012 | VFA013) Table 2 Sample capacities and system components for...
Open the catalog to page 6Performance Performance Characteristics for Vivaflow® 50 Time to concentrate up to 20x at 3 bar inlet pressure, 20°C One Cassette Three Cassettes Recovery (Direct) Yeast, 1.0 mg/mL (S. cerevisiae) 0.2 µm PES Performance Characteristics for Vivaflow® 50R Time to concentrate up to 20x at 3 bar inlet pressure, 20°C 250 mL Initial Volume Average Flux Recovery (Direct) γ Globulins, 1.0 mg/mL (150,000 MW) 100,000 MWCO Hydrosart® Time to concentrate 1 L BSA (1 mg/mL) at 3 bar inlet pressure with 10,000 MWCO Hydrosart® One Vivaflow® 50R Cassette
Open the catalog to page 8Performance Characteristics for Vivaflow® 200 Time to concentrate up to 20x at 3 bar inlet pressure, 20°C 1 L Initial Volume Average Flux Recovery (Direct) BSA, 1.0 mg/mL (66,000 MW) 5,000 MWCO PES 5,000 MWCO Hydrosart γ Globulins, 1.0 mg/mL (average 150,000 MW) 100,000 MWCO PES Yeast, 1.0 mg/mL (S. cerevisiae) 0.2 µm PES Time to concentrate dilute solute from 1 L initial volume at 3 bar inlet pressure with 10,000 MWCO PES BSA, 0.001 mg/mL Time to concentrate BSA (1 mg/mL) from 5 L initial volume at 3 bar inlet pressure with 10,000 MWCO PES BSA, 1.0 mg/mL
Open the catalog to page 9Ordering Information Quantity Pump (240 V), Easy-Load pump head (size 16), tubing, 500 mL sample|diafiltration reservoir, cassette stand, pressure indicator, T connectors and series interconnectors Pump (115 V), Easy-Load pump head (size 16), tubing, 500 mL sample|diafiltration reservoir, cassette stand, pressure indicator, T-connectors and series interconnectors Vivaflow® 50 (includes 2x cassettes with filtrate tube, size 16 peristaltic tubing, flow restrictor and fittings, and 1x series interconnector) Vivaflow® 50 complete system Vivaflow® 50R (includes 1x cassette, filtrate tube, size...
Open the catalog to page 10Pump (240 V), Easy-Load pump head (size 16), tubing and 500 mL sample|diafiltration reservoir Pump (115 V), Easy-Load pump head (size 16), tubing and 500 mL sample|diafiltration reservoir Vivaflow® 50R | 200 complete system Vivaflow® accessories Masterflex Economy Drive variable speed peristaltic pump (230 V) Masterflex Economy Drive variable speed peristaltic pump (115 V) 500 mL sample and|or diafiltration reservoir Masterflex Easy-Load pump head – size 16 Masterflex Easy-Load pump head – size 15 Vivaflow® 50 accessories for operating 2-6 cassettes T-connectors for running 2 stacks Series...
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