Vivaspin® Turbo 15 - 2 Pages

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Vivaspin® Turbo 15

Catalog excerpts

Vivaspin® Turbo 15 Allows fastest sample concentration with highest recoveries Centrifugal Concentration  Complete Recoveries Fastest Concentrations Highest Chemical Resistance Disposable Ultrafiltration device for sample concentration Introduction Vivaspin® Turbo 15 centrifugal concentrators offer the optimal solution to any concentration or buffer exchange application with their broad range of MWCOs. Highest flow rates are achieved due to their twin vertical membranes which minimize protein polarization and subsequent fouling of the membrane. Additionally, their sleek internal profile ensures maximum process speeds right down to the last 100 µl. Working Principle Centrifugation provides the vector to clear solvent and micro molecules through an ultrafiltration membrane to separate macromolecular species and solvents primarily on the basis of size. It is particularly appropriate for the concentration of macromolecules and can also be used to purify molecular species or for solvent exchange. Ultrafiltration is a non denaturing method that is more efficient, flexible and gentle than alternative processes. High Performance In a single spin, 15 ml solutions can be c ­ oncentrated up to 150x. Samples can be t ­ ypically concentrated in 10–30 minutes with macromolecular recoveries in excess of 95%. Optimized Design for Fastest Concentration Results The Vivaspin® Turbo’s optimized design, its sleek internal profile, ensures maximum process speeds right the way down to the last few micro liters. The UV joining technology allows for a smooth joint transition between membrane and plastic housing, allowing all of your valuable sample to be collected into the unique pipette friendly dead stop pocket. The ultimate in centrifugal ultrafiltration technology: – leek internal design: S Ensures maximum process speed for the complete filtration – arge twin vertical membranes: L A fouling of the membrane is avoided due to minimized protein polarization. – nique angular dead stop pocket: U The 100 µl dead stop pocket is easy to access with standard 200 µl pipette tips due to its patent pending angular design. It eliminates the risk of the sample running to dryness while allowing highest recovery of the concentrate. Optimized Choice of Materials for High Chemical Compatibility The combination of Polyethersulfone (PES), P ­ olystyrene and Polypropylene (PP) allows s ­ terilization and depyrogenization of the Vivaspin® Turbo 15 units. Polyethersulfone membranes are preferred for their low fouling characteristics, exceptional flux and broad pH range compatibility. Applications Sample preparation – ample (protein, lipid, virus, nanoparticle, S macromolecule) concentration – Desalting | Dialysis – Rebuffering Your sample is often the result of several months of research. Your sample is valuable, and Vivaspin® Turbo 15 provides highest recovery. Summary For scientists and lab technicians who need to quickly and safely concentrate biological samples of 4 ml to 15 ml up to 150 fold, S ­ artorius offers the Vivaspin® Turbo 15 u ­ ltrafilters. Unlike competitive ultrafiltration units, Vivaspin® Turbo 15 is equipped with an angular dead stop pocket, that enables reproducible and complete recoveries, while being the fastest in the market.

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Technical specifications Materials Concentrator capacity Maximum speed 3 kDa – 50 kDa Maximum speed 100 kDa Sterilization Removal of endotoxins [Depyrogenization] Body Filtrate vessel Concentrator cap Membrane Total length (concentrator insert) Total length (in tube with cap) Diameter (concentrator insert) Active membrane area Hold up volume of membrane Dead stop volume in swing out Dead stop volume in fixed angle Swing bucket rotor Fixed angle rotor (25°) 4000 + g Styrene butadiene copolymere Polypropylene Polypropylene Polyethersulfone (PES) 77 mm 118 mm 27 mm 7.2 cm2 <10 µl 100 µl 60 µl...

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