cellenion for cloning
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Automate cloning steps with ® Single cell dispenser based on acoustic handling Stem Cells LOW VOLUME, HIGH VIABILITY & PRECISION SINGLE CELL ISOLATION FOR CELL LINE DEVELOPMENT... FOR SEQUENCING... AND MUCH MORE

cellenONE® X1, an automated single cell dispensing system based on patented piezo-acoustic technology, allows precise cell deposition on a wide range of microplates (96, 384, 1536) and microwell substrates. Most dispensing and microfluidic technologies follow Poisson distribution, which leads to multiple cells per position and low efficiency. cellenONE uses software-integrated visual feedback to ensure only single cells are deposited in every position. With cellenONE® X1, cell sample is divided into droplets of identical Single cell deposited in 50 µm microwells volume. The HD camera allows...

• Choose culture media (DMEM, RPMI, Ham´s F12) Cell Sample Preparation or buffer (PBS) • Prepare a minimum volume of 2 µL, ideally around 10 µL • Optimal cell concentration below 200 cells/µL Place sample in holder Aspirate sample • Automated cell tracking Cell Mapping • Automated report generation • Choose isolation parameters • Choose type of target (96,384,1536 wp) System setup only takes a few minutes A mapping report is automatically generated, it contains: • Evaluation of ejection zone boundary • Detected cell diameter, elongation and circularity distribution • Average cell...

Results from 5x100 positions filled with single cells from four different cells samples. Single cells rate is indicated in pink, up to 99% for A549 cells; in white are empty positions and in grey, multiple cells positions. Outstanding cell viability 5% Q Single cell colony 0 Multiple cells 25% O No colony VS. Key Features cellen. NE® Only single cells dispensed High viability Suitable for most cell types % wells with single colony Limiting dilution achieves approx. 25% of wells with a single colony; but several wells will contain multiple colonies which then forces a second round of...

Accelerate Cell Line Development The main challenges associated with cell line development include maintaining good cell viability and ensuring monoclonality of isolated cells. Cloning experiment with cellenONE® showing a single CHO colony after 4 days of culture TRADITIONAL DILUTION PROCESS Limiting Dilution Limiting Dilution Best clone(s) Avoid Recloning WITH cellenONE® X1 cellenONE® X1 Best clone(s) Save on consumables and media Save time

Initial cell composition Aim was to isolate only single GFP negative cells (70%) Comparative cloning experiment: cellenONE® X1 vs. FACSAriaTM III. The aim of this experiment was to clone GFP negative cells from a mixed CHO cell suspension. Cells were isolated into antibiotic containing media that selects only cells of interest (GFP negative fraction). After 5 days of culture, colonies were counted and results summarized as shown above. FACSAriaTM III used fluorescent detection to select only GFP negative cells, as a result, theoretically, up to 100% of wells should contain single cell....

Technical information Dispensing technology: piezo acoustic drop-on-demand Dispense volume: 50-800 pL per drop Linear drives for X/Y and spindle drive for Z Resolution: 1 µm Accuracy (Absolute Position): < 10 µm Precision (Repeat Position): < 3 µm Max. speed: 100 cm/sec Spottable area (mm): x=180; y=120 (2 microtiter plates) Dimensions LxWxH (mm): 650 x 700 x 1590 -> including monitor’s arm L = 1300 mm -> with door open H = 2050 mm Weight: 205 kg Voltage: 110 V; 220 V Options & Software Related Products & Services Temperature, humidity and dew point control 2nd channel for nanoliter...

“We have been using cellenONE® in combination with our latest cell sorter, the MACSQuant® Tyto®. These two technologies offer a great combination for cell line development. The MACSQuant® Tyto® Sorter is an easy to operate instrument that gently enriches the cell population of interest, while cellenONE®’s accuracy allows for highly efficient clone selection from the enriched population. Used together, these two technologies reduced our monoclonal antibody development protocols by nearly 4 weeks.” Bastian Ackermann, Global Product Manager MACSQuant® Tyto® “HuBBBTM technology is a very...