ENHANCING LIFE Single cell isolation with cellenONE® for sequencing applications with integrated nanoliter dispenser /» 1) scATAC-Seq scMethyl-Seq scMS scYour-Seq LOW VOLUME, HIGH PRECISION & RECOVERY SINGLE CELL ISOLATION FOR SEQUENCING... AND MUCH MORE

cellenONE® X1, an automated single cell dispensing system based on patented piezo-acoustic technology, allows precise cell deposition on a wide range of microplates (96, 384, 1536) and microwell substrates. Most dispensing and microfluidic technologies follow Poisson distribution, which leads to multiple cells per position, low efficiency and biased data. cellenONE uses software-integrated visual feedback to ensure only single cells are deposited in every position. Each drop generated with cellenONE® X1 can contain: multiple cells. cellenONE® X1 only dispenses single-cell containing drops (B)...

cellenONE® X1 can now be ordered with optional fluorescence detection setup which can be used for: • Isolation of live single cells (Live/Dead) Remove dead cells from scRNA-seq libraries • Isolation of very rare single cells Only isolate single cells of interests • Cloning best expressers Boost clonal recovery and expressers levels Optional fluorescence detection comes in two levels: Sorting Features Live / Dead Very rare cells Only isolate single cells of interest A mixture of four cell populations were labelled with Hoechst 33342 (DAPI channel), CellTracker Green (FITC channel), CellTracker...

Single cells from dissociated lung cancer spheroids successfully isolated onto a microscope slide. Every position contains one single cell. Results from 5x100 positions filled with single cells from four different cells samples. Single cells rate is indicated in pink, up to 99% for A549 cells; in white are empty positions and in grey, multiple cells positions. Unlike other technologies (microdroplet, microfluidic traps), doublets can be completely excluded and only single cells are dispensed. Open and versatile platform No doublets Unseen recovery Suitable for most cell types Thanks to high definition...

Cell Sample Preparation Place sample in holder • Prepare a minimum volume of 2 |_iL, ideally around 10 |_iL • Optimal cell concentration below 200 cells/|_iL Aspirate sample Cell Mapping • Automated cell tracking and report generation • Choose isolation parameters • Select type of target (96, 384, 1536 wp. ICELL8TM chip) System setup only takes a few minutes Users can walk away during isolation process A mapping report is automatically generated, it contains: • Evaluation of ejection zone boundary • Detected cell diameter, elongation and circularity distribution • Average cell concentration Automated...

Open Workflow for Library Preparation cellenONE X1 is an open platform providing both single cell isolation and nanoliter dispensing. Such versatility allows users to automate many of the crucial steps involved in the ever growing number of single cell library preparation methods available today. Moreover, ability to work in nanovolumes and microwells allows a drastic reduction in reagents consumption and associated costs. All-in-one device Low reaction volumes Low reagent costs Single Cell Isolation Nanovolume Library Preparation Nanoliter dispensing: lysis buffer and/ or barcodes Single bead...

Rare cells isolation from cerebrospinal fluid Unseen recovery for low concentration samples for low volume samples In collaboration with the Department of Neurology at Weill Institue in San Francisco, a study performed aiming to isolate single immune cells in multiple sclerosis patient´s cerebrospinal fluid (CSF) samples for subsequent single cell RNA sequencing. The main challenge associated with such sample is the very low number of cells present in these samples which can vary from only 20 to 500 cells per 20mL of patient’s CSF. Single cell isolation with CSF collected from lumbar puncture...

DLP+ for Whole Genome Sequencing High throughput, versatile and low cost WGS Scalable whole genome sequencing of 40,000 single cells identifies stochastic aneuploidies, genome replication states and clonal repertoires Single cell WGS study performed jointly at UBC and BCCRC aimed to analyze genome heterogeneity, mutational process and clonal evolution over tens of thousands of single cells or single nuclei from a variety of tissues. The author developed DLP+, a fully integrated pipeline from wet bench to analysis Emma Laks ([email protected]), Hans Zahn1, Daniel Lai1, Andrew McPherson1, Adi Steif1,...

DLP+ for efficient Whole Genome Sequencing Direct Library Preparation Method Single cell detection and isolation Fluorescent imaging Lysis buffer addition Heat lysis Reagent addition Heat incubation Library recovery and pooling DLP+ protocol, adapted from Laks et al., BioRxiv 2018 • Compatible with cell line, dissociated mouse and human Low volume & reagent costs 0,27 US$/cell • Optimized WGS protocol for nanovolume library preparation • Open-source cloud computing pipeline for single-cell High quality library genome analytics (http://www.cellmine.org) • Pseudobulk sequence from assembled single...

Technical information Dispensing technology: piezo acoustic drop-on-demand Dispense volume: 50-800 pL per drop Linear drives for X/Y and spindle drive for Z Resolution: 1 µm Accuracy (Absolute Position): < 10 µm Precision (Repeat Position): < 3 µm HD camera for detection of cells or particles from 2µm Max. speed: 100 cm/sec Isolation area (mm): x=180; y=120 (2 microtiter plates) Dimensions LxWxH (mm): 650 x 700 x 1590 -> including monitor’s arm L = 1300 mm -> with door open H = 2050 mm Weight: 205 kg Voltage: 110 V; 220 V Options & Software Related Products & Services Temperature, humidity and...