MULTIPLEXING OF IMMUNOASSAYS OR LATERAL FLOW IN 4 STEPS Our multiplex platform represents a complete solution to create a multiplex test with your probes. You can start from scratch, or transfer existing single well or already partially multiplexed assays. The combination of miniaturization, multiplexing, production, detection and data analysis results in high performance multiplex assays that you can rely on. Feasibility Study • Microarray and assay design • Printing on preselected support • Assay incubation & detection • Detailed report Development • Assay specification • Customized surface functionalization • Evaluation of printing parameters • Protocols for printing, immobilization and assay incubation • Evaluation of detection methods • Characterization of assay performance: specificity, sensitivity, dynamic range, reproducibility • Including QC methods • Read-out MULTIPLEXED TESTS: Different needs. One personalized solution. Miniaturization / Technology sciDROP PICO • Dispensing from pico- to nanoliters • 50 - 500 pm spot size • Significant reduction of capture probe consumption (105 - 106 fold) ELISA & LFA • ELISA: transfer from whole well coating to 100 pm spot format • LFA: transfer from lines to spots • Including +/- controls and calibration features • Increased information density • Consistent or higher sensitivity Validation / Application tests - from assay controls to patient samples Production / Upscaling production / Arraying service • High quality microarrays for R&D and IVD applications • From batch to continuous production • Short production cycles from purchase order to shipment • Advanced in-process QC, 100% array control • According to ISO 9001 or 13485 Production / Contract Manufacturing sciFLEXARRAYER • R&D and high throughput production • Full in-line quality control • Automated, walk away function • Superior printing quality Printing service • Standard (sciCHIPS, sciPLEXPLATES) and custom supports (biosensors, cartridges) • Flexible model of outsourcing: from development to production • Functional quality control Multiplexing parallel detection of serveral analytes & controls in ONE cavity Supports & Immobilization • Standard formats (slides and plates): easy to use • Tailor-made surface functionalization • sciPOLY3D: biofunctionalization of unmodified polymers Incubation • sciBUFFER system: optimized for multiplexed applications and tailor-made surface functionalization • Assay protocol development & optimization Detection / Analysis / Report sciREADER CL2 • Fast read-out: 2 minutes for 96 arrays • Top and bottom lighting for opaque and transparent supports sciREADCL2 software • Applicable from R&D to routine diagnostics • Intuitive, easy-to-use software • Automated detection and analysis • Customization of array analysis and report formats We offer miniaturization and multiplexing of classic assay applications to planar array format in wells, and classic lateral flow tests to multi-analyte detection! Why choose SCIENION? • Unique feature: full package necessary for multiplexing • Modular development service & tailor-made solutions • Over 15 years of SCIENION Know-How: customized service

RELATED PRODUCTS sciMULTIPLEX Box The sciMULTIPLEX Box contains selected consumables to facilitate transfer of multiple traditional singleplex immunoassays to one multiplex immunoassay. The goal of this kit is to expedite setting up a proof-of-concept multiplex assay as a solid base for further development and validation. sciBUFFER Systems • Optimized for DNA and protein microarray assays • Compatible with all major microarray spotters sciPLEXPLATES • Microplate qualified for array based applications allow cost reduction by saving reagents • High immobilization efficiencies of biomolecules from...