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NanoLC™ 400

NanoLC™ 400

NanoLC™ 400

Product catalog summary
Overview of the NanoLC™ 400 System
The NanoLC™ 400 System by Eksigent offers unmatched flexibility for low flow LC-MS, making it ideal for workflows involving biomarker identification and small molecule quantification. It ensures accurate retention times and reproducible injections down to 200 nL, covering a broad range of flow rates.
Key Features
  • Autosampler: Provides flexible and reproducible injections with no waste.
  • Flow Accuracy: Utilizes SCIEX patented microfluidic flow control technology for consistent retention times without flow-splitting.
  • Software Integration: Controlled via SCIEX Analyst® Software.
  • Flow Rates: Features user-interchangeable plug-and-play flow modules for nanoflow (100 nL/min - 1 µL/min) and microflow (1 - 50 µL/min).
Separation Versatility
The system is designed for separation versatility with exchangeable flow modules, allowing quick transitions from nano to microflow. This flexibility supports both discovery experiments at nano flow conditions and targeted experiments at higher flow rates.
Performance and Reproducibility
  • Offers a wide flow range (100 nL/min to 50 μL/min) with improved flow rate precision for retention time reproducibility.
  • Features ultra-high performance liquid chromatography with no flow pulsations and a pressure capacity up to 10,000 PSI.
  • Ensures high retention time reproducibility, critical for quantitative proteomics workflows.
Ion Sources and System Flexibility
  • Includes rugged, reliable, and easily interchangeable ion sources for various applications and flow rates.
  • OptiFlow® Turbo V Ion Source supports a wide flow rate range with intelligent probe recognition.
Column and Chemistry Options
Offers flexibility in using any microLC or nanoLC column with a variety of chemistries to optimize assay performance. Phenomenex provides a diverse portfolio of low flow LC columns for different applications.
Support and Services
  • SCIEX Now™ Support Network offers comprehensive support, including training, workflow support, and lab enhancement services.
  • SCIEX University provides personalized learning paths and best-in-class content.
See more

Catalog excerpts

NanoLC™ 400-1

Eksigent Ekspert NanoLC ™ 400 Unmatched flexibility for low flow LC-MS

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NanoLC™ 400-2

The NanoLC™ 400 System The ideal choice for any workflow The NanoLC 400 series enables a full range of analytical workflows that drive the identification of novel biomarkers or the quantification of small molecules and biologics to advance precision medicine research. This front-end system consistently delivers accurate retention times, reproducible injection down to 200 nL and covers a broad range of flow rates. • Autosampler Provides flexible and reproducible injection down to 200 nL with no waste • Flow accuracy SCIEX patented microfluidic flow control technology, for consistent, accurate...

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NanoLC™ 400-3

Designed for separation versatility With exchangeable flow modules that extend the flow rate from nano to microflow in minutes and a high precision autosampler, the NanoLC system provides both flexibility and high throughput. Move from discovery experiments, done at nano flow conditions for the ultimate sensitivity, to targeted experiments at higher flow rates for more throughput and robustness on any mass spectrometer*. Plug-and-play flow modules enable flow rate ranges of: 100 - 1000 nL/min 1 - 10 µL/min 5 - 50 µL /min For targeted experiments, shorter columns and higher flow rates enable an...

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NanoLC™ 400-4

Superior reproducibility and reliability delivered Providing high performance microfluidic flow control Enables a wide flow range (100 nL/min to 50 μL/min) via simple exchange of flow modules. Improved flow rate precision for retention time reproducibility <0.35% RSD at 500 nL/min. •  No flow pulsations using a pneumatic amplifier •  Splitless nanoflow and microflow system •  Pressure up to 10,000 PSI Retention time (mins) Ultra high performance liquid chromatography Injection number Reproducible retention times for 56 peptides monitored in an E.coli cell lysate across 25 replicate injections...

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NanoLC™ 400-5

Exceptional ion sources Rugged, reliable, easily interchangeable ion sources are available for a wide range of applications and flow rates to suit your analysis needs. Rapid source change-over extends system flexibility with minimum downtime. All temperature, gas and electrical connections are fully integrated into the source housing—no extra lines to attach and no lost time. The system automatically detects and identifies the source without having to make hardware profile changes. OptiFlow® Turbo V Ion Source—robustness and simplicity for high sensitivity microflow analyses • ased on the trusted...

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NanoLC™ 400-6

Don’t keep your workflows under lock and key flexibility for the best performance Gain maximum flexibility by using any microLC or nanoLC column, in any column chemistry, to help ensure the best possible performing assay for all your analyte types. SCIEX affiliate, Phenomenex, offers a large and diverse portfolio of low flow LC columns for a wide range of flow rates. Select the right chemistry for your application Peptide analysis and quant Intact protein quant Small molecule quant •  Luna Omega Polar C18 Drug research panels •  bioZen XB-C18 •  Kinetex XB-C18 •  Jupiter Proteo Metabolomics • ...

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NanoLC™ 400-7

Meeting the needs for every workflow With single-gradient and dual-gradient models available—workflows from simple trap and elute to complex multi-dimensional separation are possible. Compatible with SCIEX Triple Quad™, QTRAP® and TripleTOF® systems, the NanoLC 400 series readily fits the ever-changing needs of your lab.

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NanoLC™ 400-8

SCIEX Now™ Support Network The destination for all your support needs Product and data security Increase your confidence with compliance services to help you safeguard your data, confirm data integrity and ensure system modifications can be traced. Onboarding Product and data security We register you to SCIEX Now Online, enroll you in your SCIEX University™ learning path, and send you a welcome email. SCIEX lab enhancement services apply a holistic approach to your lab to increase productivity and reduce system downtime. SCIEX Now™ SCIEX University training SCIEX University Best-in-class content,...

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.