PA 800 plus Catalog Built on 20 years of innovation and leadership in the field of capillary electrophoresis, Beckman Coulter’s PA 800 plus provides solutions for protein purity analysis, charge heterogeneity assessment, glycan characterization and small molecules. Systems, software, assay kits, capillaries, reagents, and system supplies for the PA 800 plus can be found here. Systems/Software Part Number PA 800 plus Pharmaceutical Analysis System The PA 800 plus Pharmaceutical Analysis System provides a comprehensive, automated, and quantitative solution for the characterization and analysis of proteins. Innovative system design ensures dependable operation and durability. The PA 800 plus application menu includes SDS-gel molecular weight analysis, charge heterogeneity analysis utilizing advanced capillary isoelectric focusing technology, and carbohydrate profiling for the assessment of glycoprotein microheterogeneity. Modular design provides for the simple interchange of detectors. The PA 800 plus System includes: • High-resolution separation module • High-sensitivity UV, PDA, and solid-state laser-induced fluorescence detection • Sample storage temperature control • High-speed system controller with 32 Karat Version 9.0 and PA 800 plus software • Validated applications for SDS-MW, IgG, cIEF, and glycan analysis • Starter kit containing necessary hardware supplies to operate the system • Installation qualification and operation qualification of the system • System application installation and training PA 800S plus Pharmaceutical Analysis System The PA 800S plus Pharmaceutical Analysis system provides an automated and quantitative solution for the analysis of protein purity and molecular weight utilizing SDS-gel capillary electrophoresis. The innovative system design ensures dependable operation and durability. Additional detectors are available for future upgrades. The PA 800S plus System includes: • High-resolution separation module • High-sensitivity PDA detection • Sample storage temperature control • High-speed system controller with 32 Karat Version 9.0 and PA 800 plus software • Validated applications for SDS-MW and IgG Purity and Heterogeneity • Starter kit containing necessary hardware supplies to operate the system • Installation Qualification and Operation Qualification of the system • System application installation and training Test Kit, Capillary Performance Includes: • Capillary Regenerator Solution A (Part Number 338424) • Performance Test Mix B (Part Number 501333) • Capillary Performance Run Buffer A (Part Number 338426) • Instructions

Assay Kits Part Number IgG Purity and Heterogeneity Assay The IgG Purity and Heterogeneity Assay has been developed for researchers employed in industrial biotechnology, who are developing and manufacturing IgG reagents for research, diagnostic and therapeutic use. This assay has been specified to assess the purity and heterogeneity of IgG reagents in both a reduced and non-reduced state. The methodology involves heat-denaturing at a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS, and separating these proteins by size using high resolution capillary gel electrophoresis...

Assay Kits (continued) Part Number Carbohydrate Labeling and Analysis Kit The PA 800 plus Carbohydrate Labeling and Analysis Chemistry contains the reagents and separation capillary required to label, separate and quantify oligosaccharides and monosaccharides released from glycoproteins. Following release (enzymatic or chemical) from proteins, glycans are labeled with a fluorophore, APTS, at the reducing terminus by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides...

Assay Kits (continued) Part Number The Beckman Coulter Anion Analysis Kit contains the supplies necessary for separation and quantitation of inorganic anions and organic acids using the P/ACE MDQ or PA 800 capillary electrophoresis systems. Each kit yields approximately 500 tests. Includes: • Anion Coating, 38.5 mL • Anion Separation Buffer, 115.5 mL • Conditioner — Na, 38.5 mL • Anion Acid Rinse, 38.5 mL • Anion Internal Standard, 20 mL • Anion Organic Test Mix, 3.5 mL • Anion Inorganic Test Mix, 3.5 mL • Capillaries, 50 cm, 75 micron ID, (pkg of 3) (Part Number 338454) • Rinse Solution, 192.5...

Capillaries (continued) Part Number Bare Fused-Silica Capillary - 75 μm ID, 375 μm OD, 5 m length Bare fused-silica capillary 75 micron internal diameter, 375 μm OD, 5 meter length. Bare Fused-Silica Capillary - 100 μm ID, 375 μm OD, 5 m length Bare fused-silica capillary 100 micron internal diameter, 375 μm OD, 5 meter length. Bare Fused-Silica Capillary - 20 μm ID, 375 μm OD, 37 cm (pkg of 3) Capillary for use in PA 800 series and P/ACE MDQ Capillary Cartridges for separation and analysis of a variety of samples, including pharmaceuticals, industrial chemicals, chiral drugs, nucleic acids,...

Capillary Cartridges and Supplies Part Number Includes: • Cleaving stone • Capillary length tool • O-ring Installation tool • O-ring Aperture (qty 10) • Tweezers (plastic) • Instructions for Rebuild Kit OpCal Cartridge Blank cartridge used for calibration of the PDA detector. Consists of 20-, 30-, 40-, and 50 cm lengths of cartridge coolant tubing and all connectors. Cartridge Coolant Tube Kit, Includes: • Cartridge coolant tubing (100 cm total length) • Tubing-molded nuts (qty 2) • Ferrule (qty 2) • O-ring (qty 2) Cartridge Assembly Kit, Blank Includes: • Cartridge body • 100 x 800 μm aperture...

Capillary Cartridges and Supplies (continued) Cartridge Aperture O-Ring. Part Number A replacement filter for use with UV detection. A custom application filter for use with UV detection. A replacement filter for use with UV detection. A custom application filter for use with UV detection. A custom application filter for use with UV detection. A replacement filter for use with UV detection. A custom application filter for use with UV detection. A replacement filter for use with UV detection. A custom application filter for use with UV detection. A custom application filter for use with UV detection....