PA 800 plus Pharmaceutical Analysis System Brochure (BR-12431)
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PA 800 plus Pharmaceutical Analysis System Now sold through SCIEX Separations

The following Application Information Bulletins are available on CELeader.com. Separation of Fucosylated and non-Fucosylated Carbohydrates Associated with Monoclonal Antibodies using Capillary Electrophoresis (AIB IB-15285A) This bulletin describes how CE can be used to successfully separate and quantify a wide array of N-linked oligosaccharides associated with MAbs. Capillary Electrophoresis in Quality Control: PART I: Application for Therapeutic Proteins (AIB T-14326A) This bulletin illustrates an overview of CE in a modern quality control laboratory. A Robust cIEF Method: Intermediate...

Automated and Quantitative Analysis of Biologics The PA 800 plus Pharmaceutical Analysis System was designed in collaboration with biopharmaceutical development and QC groups. This platform provides analysts with robust and easy-to-use characterization, integrating quantitative, qualitative and automated solutions for protein purity, charge isoform distribution and glycan analysis. During the design of the PA 800 plus, Beckman Coulter emphasized assay portability, enhancing the overall system utility in multi-user, multi-instrument facilities. Automated applications provide reproducible and...

PA 800 plus Pharmaceutical Analysis System PROTEIN PURITY CHARGE HETEROGENEITY CARBOHYDRATE PROFILING Quantitative Protein Purity Analysis with SDS-Gel Capillary Electrophoresis The PA 800 plus Pharmaceutical Analysis System automates size separation of proteins and provides high-resolution, quantitative data, as compared to traditional labor-intensive slab gels. The CE SDS-gel application has become the gold standard for protein purity analysis in biopharmaceutical laboratories. Denatured proteins can be reduced or left intact for separation and subsequent analysis. The IgG Purity and...

IgG Purity and Heterogeneity Assay The PA 800 plus IgG Purity and Heterogeneity Assay Kit (p/n A10663) was designed in collaboration with biopharmaceutical analysts developing and manufacturing therapeutic MAb molecules. Assay methodology involves heat denaturation of IgG in the presence of SDS, followed by size separation using high-resolution capillary gel electrophoresis technology. • Detection of impurities below 0.1% More information on the SDS-gel application is available in the following Beckman Coulter application information bulletin: “Assay of IgG Purity and Heterogeneity Using...

PA 800 plus Pharmaceutical Analysis System PROTEIN PURITY CHARGE HETEROGENEITY CARBOHYDRATE PROFILING Quantitative Protein Charge Heterogeneity Accurate determination of a protein’s charge heterogeneity helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of proteins separated by isoelectric point (pI). The PA 800 plus Pharmaceutical Analysis System automates advanced cIEF technology to achieve high precision and quantitative separations. Use of optimized methods and synthetic pI markers attains the highest...

In clEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pi is formed. Comprehensive optimization of multiple assay parameters has been performed. Successful transfer and implementation of characterization assays between laboratories is based on a method's ability to minimize environmental and operator variability. An important indicator of the necessary robustness is intermediate precision. Performing advanced clEF on the PA 800 plus system...

Pharmaceutical Analysis System PROTEIN PURITY CHARGE HETEROGENEITY CARBOHYDRATE PROFILING Carbohydrate Profiling Glycosylation on a protein is an important post-translational modification that can affect its function, clearance and stability. The PA 800 plus Pharmaceutical Analysis System simplifies the complex process of profiling carbohydrates associated with glycoproteins. By providing specific and accurate quantitation of glycosylation levels, differences in glycoform quantities G10 Gl 1 Glucose Ladder Standard Key benefits to carbohydrate profiling using capillary electrophoresis...

Oligosaccharide Analysis Simplified Following endonuclease cleavage from the protein, oligosaccharides are specifically labeled with amino-pyrene-trisulfonic acid (APTS) by reductive amination. This analysis is performed directly from With APTS derivatization and LIF detection, each sugar yields the same detector response, so their relative quantities can be directly compared. Complex oligosaccharide Polypeptide sequence Oligosaccharide distribution associated with a protein yields a fingerprint that can be used in protein identification. The PA 800 plus provides easy, quantitative and...

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The PA 800 plus software quickly guides users from set-up through routine system operation. Large icons provide intuitive guidance for navigation, while on-screen cues indicate system progress at a glance. Insightful Help menus and descriptive system prompts further simplify operator learning, making transfer of PA 800 plus technology to other analysts easier. Ultimately, using PA 800 plus software is as easy as 1, 2, 3. Acquire data Use the Help and video menu as needed. PA 800 plus software features include: • Automated sequence table and reagent calculations • Validated applications for...

PA 800 plus Pharmaceutical Analysis System SOFTWARE SYSTEM A Robust Solution for Demanding Research Requirements The PA 800 plus offers dependable, accurate determination of protein purity, heterogeneity and identity. To create it, Beckman Coulter collaborated with biopharmaceutical development and Automated Sample Introduction The PA 800 plus system offers fully automated methods and extended sample-handling capability for walk-away operation. Sampling can be performed using 1.8 ml universal vials, 96-well plates and micro vials. Precision-molded polymethylpentene universal vials...