Overview
Sentinel Diagnostics, based in Milan, Italy, has been developing innovative in vitro diagnostic (IVD) devices for over 30 years. The company focuses on providing reliable clinical chemistry and immunochemistry solutions to clients in over 50 countries.

Iron Analysis Method
The method involves freeing iron (Fe+++) from its carrier protein transferrin, reducing it to Fe++ ions, and forming a colored complex with Ferene™. This method eliminates copper ion interference and is suitable for serum and plasma samples. Key features include a sensitivity of 5.0 µg/mL, linearity up to 1000 μg/dL, and a 24-month stability.

Zinc Analysis Method
Zinc (Zn++) forms a stable colored complex with 5-Br-PAPS, with interference from iron or copper ions eliminated. This method is applicable to serum, heparin-plasma, and seminal fluid samples, with a sensitivity of 5 µg/dL and linearity up to 2000 μg/dL. The stability is 30 months.

Copper Analysis Method
Copper (Cu++) is freed from ceruloplasmin and forms a stable complex with DiBr-PAESA. This method is applicable to serum, heparin-plasma, and urine samples, with a sensitivity of 3 µg/dL and linearity up to 500 μg/dL for serum and plasma, and 20 μg/dL for urine. The stability is 36 months.

Analytical Procedures
Each element (iron, zinc, copper) has a specific analytical procedure involving sample mixing, incubation, and reading at specified wavelengths and temperatures. The procedures are designed for rapid use and excellent correlation with atomic absorbance methods.

Product Specifications
The document lists various reagent kits and controls for iron, zinc, and copper analysis, including their sizes and references. These kits are compatible with common analyzers like AU series, ADVIA series, Roche Modular, and others.

Conclusion
Sentinel Diagnostics provides robust and reliable methods for the analysis of essential trace elements, ensuring optimal performance and health benefits through accurate laboratory analysis.