AXIMA Performance™ - application-centric solutions Proteomics suite Designed with the flexibility to adapt to users’ workflow: from single sample manual acquisition to fully automated data-dependent peptide mass fingerprinting and MS/MS for protein identification. Peptide mass fingerprints are acquired and subjected to an optional integrated Mascot® database search. User defined acceptance limits for PMF-based protein identification. Data-dependent MS/MS: using the results of the PMF search, MS/MS may be performed on ions that matched to the top ranked protein hit (confirmation MS/MS), in addition to those that were not (investigation MS/MS). Batch searching of these MS/MS spectra is then performed automatically to provide further and higher confidence protein identification. Data may be reprocessed and resubmitted for database searching at a later time to provide additional information. % 100 90 80 70 60 50 LC-MALDI/Relative Quantitation experiments AXIMA Performance TM The Axima Performance™ provides total support for LC-MALDI based experiments. The software suite allows the fully automated acquisition of LC separated samples deposited onto MALDI targets and the subsequent identification of proteins via MS/MS of the peptides detected. The workflow automatically provides a provisional intensity map of all sample spots across the target to assess the distribution of peptides and identify the position of the apex of the chromatographic peaks.These are utilised to generate a candidate list and MS/MS data acquired for all discrete peptide ions. Exclusion lists are used to remove known contaminants or high abundance peptides. All data is then subjected to an integrated Mascot® search. Quantitation may also be performed on MS/MS based chemistries, using proprietary software providing valuable information regarding both the identity and relative amount of protein in a complex sample. Low sample consumption allows multiple spectra to be acquired from the same spot increasing the amount of MS/MS data obtained. Axima Performance - a highly flexible research grade mass spectrometer – from high energy MS/MS of proteomics and other biological and organic samples to uncompromised analysis of high mass intact proteins. True high energy MS/MS – CID with a laboratory frame collision energy of 20keV Optimal precursor ion selection resolution using revolutionary gating technology Outstanding sensitivity – uncompromised design, to ensure no MS/MS signal is discarded Biomarker Recognition/Tissue Imaging 40 30 20 10 0 3480 3485 3490 3495 3500 3505 M ass/Charge 3510 3515 3520 3525 MS spectrum of Insulin B chain demonstrating resolution >20,000 (FWHM) % 100 %Int. 90 100 90 80 80 70 60 50 70 40 30 20 10 0 60 80 100 120 140 160 1 [c ].1 2 3 M ass/Charge 50 40 30 20 10 0 1 [c ].1 2 100 200 300 400 500 600 700 800 900 1000 1100 M a s s /C h a r g e Typical example of an MS/MS spectrum obtained by automated acquisition % 100 90 80 70 60 50 40 30 20 10 0 1500 1520 1540 1560 1580 1600 Mass/Charge 1620 1640 1660 Peptide MS spectrum demonstrating attomole level sensitivity The Axima Performance™ allows full integration with the CHIP-1000™ matrix delivery system to allow seamless tissue imaging experiments Reproducible automated matrix delivery directly to the sample surface using the CHIP-1000™. Seamless export of sample location to the Axima™ MALDI mass spectrometer Automated acquisition of MS data – profiling or imaging Visualization of the spatial distribution of components of interest via intensity mapping software Easy export of data to alternative processing packages, including BioMAP Additional application packages The system’s flexibility and uncompromised linear mode performance also lends itself to alternative applications such as quality control. Launchpad™ software offers a module performing fully automated QC analysis of large numbers of oligonucleotides, peptides or small molecules, complete with a report indicating the presence or absence of a target compound, an estimate of the purity and occurrence of known contaminants, adducts or truncated/extended analogues. Polymer applications are also fully catered for using specifically developed polymer analysis tools suitable for use with both polymers and co-polymers. Mascot® is a registered trademark of Matrix Science Ltd., London, UK. AXIMA™, AXIMA Performance™, LAUNCHPAD™, INTELLIMARQUE™, MonoPULSE™, Low Mass Zoom (LMZ)™ and Optimass™ are trademarks of Kratos Analytical Ltd., Manchester, UK. Xcise™, Accuspot™ and ChIP™ are trademarks of Shimadzu Corporation, Kyoto, Japan. www.shimadzu-biotech.net [email protected] E & OE Ref: MO300 Issue 1

AXIMA Performance™ - the next generation in MALDI CID MS/MS A high performance MALDI TOF-TOF mass spectrometer utilising state of the art high energy MS/MS, delivering unparalleled flexibility, in a robust and reliable research grade system. Essential features providing confident results This next generation design MALDI system delivers all of the features expected of an AXIMA™ series mass spectrometer: High resolution MS data in reflectron mode for more accurate and confident peptide mass fingerprinting (PMF) and complex mixture analysis. Uncompromised linear mass range and sensitivity. Near-axis...