bringing analysis to life Mascot® is a registered trademark of Matrix Science Ltd., London, UK. AXIMA™, AXIMAAssurance™, AXIMA Confidence™, AXIMA Performance™, AXIMA Resonance™, INTELLIMARQUE™ and PTM Finder™ are trademarks of KratosAnalytical Ltd., Manchester, UK. Accuspot™ and ChIP™ are trademarks of Shimadzu Corporation, Kyoto, Japan. AXIMA Resonance AXIMA Resonance™ - a unique mass analyzer combining the simplicity of MALDI, the power of MSn and accuracy generation of structural and sequencing i Excellent precursor ion selection Outstanding sensitivity • Innovative software tools to aid bringing analysis to life

A unique MALDI QIT TOF MS designed for the structural characterization and sequencing of biomolecules not just mass measurement. Quadrupole ion trap - True MS for structural studies n Variable energy CID control on the fly High resolution precursor ion selection – up to 1000 FWHM High mass resolution and mass accuracy across n MS and MS analyses Outstanding sensitivity - uncompromised design, to ensure highly efficient trapping functionality Low sample consumption- allowing many more MSn experiments to be performed on the same sample spot Variable repetition rate N2 laser Manual or fully automated...

High performance with an innovative design Advanced MS performance n Zoom view The unique characteristics of the instrument allow controlled, flexible and complete searchable fragmentation for target identification and characterization. The unique combination of MALDI and quadrupole ion trap allows: generation of ions using a number of different matrices positive and negative ionization modes, switchable via software in seconds (M+H)+ (M+2H)2+ Des-Acyl Ghrelin (human), an extremely fragile molecule, in MS mode demonstrating excellent resolution and the presence of the doubly charged ion Zoom...

MS – using multiple fragmentation stages for structural elucidation n High performance MS/MS mode: 603-QQQHLFGSNVTDCSGNFCLFR-623 m/z 4136 The combination of a QIT and high performance reflectron allows superior resolution and excellent sensitivity MS/MS spectra.The AXIMA Resonance™ permits MS/MS spectra of a wide range of analytes from pharmaceutical compounds, through peptides, glycans, lipids and polymers. 1160.26 +b12-18 90 70 y8 y10 y11 Pep -18 Pep -18 203 120 Pep -18 162 162 Pep -18 162 162+18 203 Pep -18 1069.30 800 1000 1200 )* 1400 1600 2200 2400 2600 Mass/Charge )* 3000 3958.75 3229.54...

Software tools to empower complemented by a suite of software tools allowing many different types of • Fully enabled for proteomics experiments - Intellimanque™ software suite for automated data dependent peptide mass fingerprinting and MS/MS of peptides with optional incorporated Mascot database searching • LC-MALDI software allowing confident identification of off-line separated complex • Tissue imaging suite - allows full integration with the CHIP™ tissue sample preparation device, automated acquisition of data and interrogation using proprietary visualization software or automated export...

Post translational modification analysis - AXIMA Resonance™ provides total support for many types of PTM analysis using the PTM Finder™ software suite of tools - software that facilitates the user's workflow when dealing with MS/MS data sets. Phosphorylation, glycosylation, oxidation, methylation and acetylation amongst others can be characterized. An MS/MS data set is interrogated for the presence of user defined post translational modifications following an initial Mascot search:- • Peptides that are unmatched in this first pass are subjected to a second search using different search parameters....