GEXSCOPE
2Pages

Product Profile From single cell multi-omics to precision medicine GEXSCOPE® Single Cell RNAseq Library kits GEXSCOPE® Single Cell RNAseq kits have all key components required to go from any sample to ready-to-use sequencing library: SCOPE-chip™ for single cell partition, AccuraCell® beads for barcoding and capturing mRNA and enzyme master mixes for transcriptome amplification and library construction. For tissue samples, the GEXSCOPE® Tissue Kits also contain sCelLiVE™ Tissue Preservation Buffer and Tissue Dissociation Master Mix, circumventing the need to process tissue samples immediately after resection. GEXSCOPE® Kits offer: 1. Complete solution from sample to library within one workday 2. Flexible SCOPE-chip™ configuration for 500 - 30,000 cells per sample 3. Unique sCelLiVE™ tissue processing reagents to maximize cell viability GEXSCOPE® Kits enable a streamlined workflow for single cell RNA sequencing library preparation from either tissue samples or cells within a workday (Figure 1). The protocol can be performed either manually or automated on the Singleron MatrixTM instrument. The microfluidic SCOPEchipTM contains microwells that can easily partition single cells into individual wells. Two different configurations, standard and high density (HD) SCOPE-chipsTM, can be used to obtain sequencing results of up to 10,000 or 30,000 single cells per sample, respectively. The ultra-high throughput HD format can be used to detect and analyze rare cell populations in a sample (Figure 2). *mRNA is linked to barcoded beads Figure 1: Streamlined workflow: sample to library within one workday Figure 2: Ultra-high throughput single cell sequencing enabled by SCOPE-chip™ HD. A complete mouse embryo was processed with GEXSCOPE® Single Cell RNAseq Library Kit (HD). Cells were loaded onto two SCOPE-chips (HD) and 42,641 cells from mouse embryo, showing cell population as

From single cell multi-omics to precision medicine GEXSCOPE® kits: Single Cell Library Construction. Any Sample. Sequencing single cells from tissue samples typically requires freshly resected tissues, making it challenging to plan experiments. This is a bottleneck particularly in a clinical setting and where samples from different locations are collected and processed in a central laboratory. sCelLiVE™ Tissue Preservation Solution has a buffer formulation that mimics physiological conditions to maintain cell viability for up to 72 hours at 2-8℃. Samples are easily shipped and stored,...