High Density Microfluidic SCOPE-chip™
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Technical Note High Density Microfluidic SCOPE-chip™ Boost cell throughput on one chip This technical note describes Singleron’s High Density (HD) Microfluidic SCOPE-chip™, a microwell chip for single-cell dispersion, mRNA capture, and barcoding. Its performance is evaluated focusing on coverage of all cell types, consistency, and doublet rates. Overview High-throughput single-cell sequencing technologies have advanced rapidly during the past decade. Nowadays, researchers and clinicians apply this technology to improve the understanding of mechanisms and treatment options of a variety of diseases. To understand the complex biology and to detect important rare cell populations more sensitively, the number of cells that need to be analyzed in these single-cell studies steadily increases. To address such needs, Singleron has developed a unique high-density (HD) microfluidics SCOPE-chip™ for high throughput processing of up to 30,000 cells on one chip. High-throughput – process up to 30,000 cells on one chip Reliable – low occurrence of doublets Flexible – available as manual and

Technical Note Workflow and Performance The greater the number of cells captured, the more types of cells can be detected, and the more accurate is the annotation of differences between cell subpopulations. Specifically addressing this need, Singleron has developed a high-density (HD) SCOPE-chip (Figure 1) with 250,000 microwells, which can capture up to 30,000 cells on a single chip. Thus, the HD SCOPE-chip technology accelerates cell capture rates at low doublet occurrence. For a comprehensive comparison of the HD SCOPE-chip configuration and performance with two products offered by a...

Technical Note High Consistency To evaluate the consistency of data, when processing samples on the HD chip, three mouse testis samples were processed and analyzed following the standard GEXSCOPE workflow, simultaneously. At a sequencing depth of 13,200 reads/cell, the average number of cells detected was 21,289 cells, the mean gene number was 1350 genes, and the average number of total genes was 31,116 genes. Among the eight cell types identified within mouse testis samples, including spermatids, leydig cells, spermatocytes, round spermatids, spermatogonia, sertoli cells, macrophages, and...

Technical Note Capture of Rare Cell Types To investigate the ability of Singleron’s HD SCOPEchip to capture rare cell types, mouse embryonic cells were processed on two HD SCOPE-chips, then libraries were pooled and analyzed at a sequencing depth of 21,900 reads per cell. A total of 42,628 cells was detected with a median of 1677 genes per cell and 31,917 genes, cumulatively. According to the cell clustering and cell type annotation, 26 different cell types were detected in the mouse embryo tissue sample (Figure_3). Mesoderm cells were the most abundant cell type, making up 32.71% of the...

Technical Note Low Doublet Rate In order to evaluate occurring doublet rates, Singleron’s HD SCOPE-chip technology was compared to another commercially available platform (Figure 4). While higher numbers of captured cells come with an enormous increase in data output, doublet rates only moderately rise. Thus, Singleron’s HD SCOPE-chip technology outperformed the other technology. To match the performance measured of the HDSCOPE-chip with occurring doublet rates, a 293T+ mouse testicle cell line was analyzed (Table_4). This data set shows that 2.68% doublets occur, when capturing 14.929...

Technical Note Product described in this technical note Catalog GEXSCOPE® Single Cell RNA Library Kit Cell for Matrix HD GEXSCOPE® Single Cell Human Immuno-TCR Kit Cell for Matrix HD Related products Catalog CLindex™ Sample Multiplexing Kit Contact us Phone：+49 221 16824777 E-mail：info@singleronbio.com Singleron Biotechnologies GmbH; Gottfried-Hagen-Strasse 60, 51105 Cologne, Germa