Competent Cell Mix then Spread - Saving Time ● High Transformation Efficiency > 1 x 10 8- 10 9cfu/μg pUC19 DNA Ideal for all cloning and library preparation ● Time Saving, Easy-to-Use Transformation needs only 20 seconds to 6 minutes. Heat shock and recovery procedures optional. ● No Expensive Equipment needed No need for an expensive electroporator and disposable cuvettes E-mail: [email protected] | www.smob

Time-saving Transformation Protocol O Quickly thaw the Champion™ Competent Cells with hand (body temperature), warm water bath or running tap water about 10~20 seconds until 1/3~1/2 volume is thawed. Add less than 10% of competent cell volume DNA (ex. ligation products) to cells. Vortex 1 sec or tap the tube with finger to mix well. Incubate on ice for 0~10 min. (The ice-incubation will slightly increase efficiency) Plate the bacteria/DNA mixture onto a pre-warmed (room temperature to 37°C) and dried selective LB agar plate (LB + antibiotic). Incubate the plates at 37°C until the colonies are...