FIREScript Reverse Transcriptase
4Pages

FIREScript Reverse Transcriptase ® Expand Your Horizons Teaduspargi 9, 50411 Tartu, Estonia

FIREScript® reverse transcriptase FIREScript® is an improved version of Murine Moloney Leukemia Virus (M-MLV) reverse transcriptase (RT) with exceptional stability at room temperature, improved thermostability, substantially faster synthesis rates and higher sensitivity compared to the wild-type M-MLV RT. FIREScript® is used for first strand cDNA synthesis from total RNA or purified mRNA and is suitable for wide range of reaction temperatures. Stable at room temperature for 30 days Suitable for working between 37-60°C cDNA synthesis completed in 15 minutes Detecting total RNA amounts from...

Stable at room temperature for 30 days Proprietary "stability TAG" significantly improves stability at room temperature, making it the only product on the market which is safe to be handled without ice during reaction set-up as well as during shipping across the world. FIREScript® is number one choice for field-work as well as for high-throughput screening platforms. Amplification Plot Melt Curve Plot Figure 2. Human total RNA (10 ng) was converted into cDNA using oligo-dT primers and FIREScript® reverse transcriptase (10 U/μl). Real-time PCR was performed using ß2M primers and HOT FIREPol®...

• First strand cDNA synthesis for 2-step RT-PCR and RT-qPCR • Reverse transcription at high temperature to reduce effects of secondary structure • Wierz, J., Gaube, P., Klebsch, D., Kaltenpoth, M., & Flórez, L. (2021). Transmission of Bacterial Symbionts With and Without Genome Erosion Between a Beetle Host and the Plant Environment. Frontiers In Microbiology, 12. • Lin, J., Chung, P., Dai, S., Tsai, W., Lin, Y., & Kuo, Y. et al. (2021). Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model. PLOS...